Use of Cell Separation to Analyze the Mechanisms of Action of GnRH

  • Jacques Duval
  • Michèle le Dafniet
  • Laurence Benoist
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

We are at present engaged in the study of the molecular mechanisms by which gonadotropin-releasing hormone (GnRH) stimulates the release of the gonadotropins and how this promoted release can be modulated by sex steroids. Two questions are of particular interest to us: (1) Is cyclic AMP synthesis or degradation primarily affected by the hypothalamic factor in the gonadotrophs? (2) Are the receptors for sex steroids equally or selectively distributed among the different cell categories? To answer these questions, we were led to use fractionated cells.

Keywords

Sucrose Albumin Estrogen Sedimentation Trypsin 

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References

  1. Hymer, W. C., Evans, W. H., Kraicer, J., Mastro, A., Davis, J., and Griswold, E., 1973, Enrichment of cell types from the rat adenohypophysis by sedimentation at unit gravity, Endocrinology 92: 275.PubMedCrossRefGoogle Scholar
  2. Valotaire, Y., Le Guellec, R., Kercret, H., Guellaen, G., and Duval, J., 1975, Induction of rat pituitary thymidine kinase: Another physiological response to estradiol in the male?, Mol. Cell. Endocrinol. 3: 117.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Jacques Duval
    • 1
  • Michèle le Dafniet
    • 1
  • Laurence Benoist
    • 1
  1. 1.Equipe de Recherche Associée au C.N.R.S. No. 070567, Laboratoire de Neurobiologie MoléculaireUniversité de RennesRennesFrance

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