Use of Cell Separation to Analyze the Mechanisms of Action of GnRH
We are at present engaged in the study of the molecular mechanisms by which gonadotropin-releasing hormone (GnRH) stimulates the release of the gonadotropins and how this promoted release can be modulated by sex steroids. Two questions are of particular interest to us: (1) Is cyclic AMP synthesis or degradation primarily affected by the hypothalamic factor in the gonadotrophs? (2) Are the receptors for sex steroids equally or selectively distributed among the different cell categories? To answer these questions, we were led to use fractionated cells.
KeywordsThymidine Kinase Pituitary Cell Cell Separation cAMP Content Hormone Content
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