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Functional Heterogeneity of Separated Dispersed Gonadotropic Cells

  • Carl Denef
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

It is well documented that the gonadotropic cell population of the pituitary gland is morphologically heterogeneous. Histochemical staining reactions (Herlant, 1960, 1964) and electron-microscopic examinations (Farquhar and Rinehart, 1954; Barnes, 1962; Yoshimura and Harumiya, 1965; Kurosumi and Oota, 1968) have revealed the presence of two different cell types. One contained two populations of secretory granules the size of which was about 200 and 700 nm, respectively, and was believed to secrete follicle-stimulating hormone (FSH). The other was smaller, contained only one type of secretory granules with a size of 250 nm, and was thought to secrete luteinizing hormone (LH). However, after immuno-chemical methods became available to demonstrate directly the hormones in the cell, it became evident that there was no separate location of the two gonadotropins in a given cell type (see the review by Girod, 1977). Several investigators showed that each of these cell types was immuno-reactive to anti-LH antiserum (Tougard et al., 1973; Moriarty, 1975) as well as to anti-FSH antiserum (Tixier-Vidal et al., 1975b; Moriarty, 1976).

Keywords

Luteinizing Hormone Follicle Stimulate Hormone Pituitary Cell Anterior Pituitary Cell Functional Heterogeneity 
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References

  1. Allanson, M., and Parkes, A. S., 1966, Cytological and functional reactions of the hypophysis to gonadal hormones, in: Marshall’s Physiology of Reproduction, Vol. 3 (A. S. Parkes, ed.), p. 147, Longmans, Green, London.Google Scholar
  2. Apfelbaum, M. E., and Taleisnik, S., 1976, Interaction between estrogen and gonadotro-phin-releasing hormone on the release and synthesis of luteinizing hormone and follicle-stimulating hormone from incubated pituitaries, J. Endocrinol. 68: 127.PubMedCrossRefGoogle Scholar
  3. Baker, B. L., Reel, J. R., Van Dewark, S. D., and Yu, Y.-Y., 1974, Persistence of cell types in monolayer cultures of dispersed cells from the pituitary pars distalis as revealed by immunohistochemistry, Anat. Rec. 179: 93.PubMedCrossRefGoogle Scholar
  4. Barnes, B. G., 1962, Electron microscope studies on the secretory cytology of the mouse anterior pituitary, Endocrinology 71: 618.PubMedCrossRefGoogle Scholar
  5. Blackwell, R. E., and Guillemin, R., 1973, Hypothalamic control of adenohypophyseal secretions, Annu. Rev. Physiol. 35: 357.PubMedCrossRefGoogle Scholar
  6. Bowers, C. Y., Currie, B. L., Johansson, K. N. G., and Folkers, K., 1973, Biological evidence that separate hypothalamic hormones release the follicle stimulating and luteinizing hormones, Biochem. Biophys. Res. Commun. 50: 20.PubMedCrossRefGoogle Scholar
  7. Debeljuk, L., Khar, A., and Jutisz, M., 1978, Effects of gonadal steroids and cycloheximide on the release of gonadotrophins by rat pituitary cells in culture, J. Endocrinol. 77: 409.PubMedCrossRefGoogle Scholar
  8. Denef, C., Hautekeete, E., and Rubin, L., 1976, A specific population of gonadotrophs purified from immature female rat pituitary, Science 194: 848.PubMedCrossRefGoogle Scholar
  9. Denef, C., Hautekeete, E., De Wolf, A., and Vanderschueren, B., 1978a, Pituitary basophils from immature male and female rats: Distribution of gonadotrophs and thyrotrophs as studied by unit gravity sedimentation, Endocrinology 103: 724.PubMedCrossRefGoogle Scholar
  10. Denef, C., Hautekeete, E., and Dewals, R., 1978b, Monolayer cultures of gonadotrophs separated by velocity sedimentation: Heterogeneity in response to luteinizing hormone-releasing hormone, Endocrinology 103: 736.PubMedCrossRefGoogle Scholar
  11. Döhler, K. D., and Wuttke, W., 1975, Changes with age in levels of serum gonadotropins, prolactin, and gonadal steroids in prepubertal male and female rats, Endocrinology 97: 898.PubMedCrossRefGoogle Scholar
  12. Drouin, J., and Labrie, F., 1976, Selective effect of androgens on LH and FSH release in anterior pituitary cells in culture, Endocrinology 98: 1528.PubMedCrossRefGoogle Scholar
  13. Drouin, J., Lagacé, L., and Labrie, F., 1976, Estradiol-induced increase of the LH responsiveness to LH releasing hormone (LHRH) in rat anterior pituitary cells in culture, Endocrinology 99: 1477.PubMedCrossRefGoogle Scholar
  14. Drouin, J., Lavoie, M., and Labrie, F., 1978, Effect of gonadal steroids on the luteinizing hormone and follicle-stimulating hormone response to 8-bromo-adenosine 3′,5′-mon-ophosphate in anterior pituitary cells in culture, Endocrinology 102: 358.PubMedCrossRefGoogle Scholar
  15. Epstein, M. L., and Gilula, N. B., 1977, A study of communication specificity between cells in culture, J. Cell Biol. 75: 769.PubMedCrossRefGoogle Scholar
  16. Epstein, Y., Lunenfeld, B., and Kraiem, Z., 1977, The effects of testosterone and its 5α-reduced metabolites on pituitary responsiveness to gonadotrophin-releasing hormone (Gn-RH), Acta Endocrinol. 86: 728.PubMedGoogle Scholar
  17. Farquhar, M. G., and Rinehart, J. F., 1954, Electron microscopic studies of the anterior pituitary gland of castrate rats, Endocrinology 71: 618.Google Scholar
  18. Farquhar, M. G., Skutelsky, E. H., and Hopkins, C. R., 1975, Structure and function of the anterior pituitary and dispersed pituitary cells: In vitro studies, in: The Anterior Pituitary (A. Tixier-Vidal and M. G. Farquhar, eds.), p. 83, Academic Press, New York.Google Scholar
  19. Fletcher, W. H., Anderson, N. C.,Jr., and Everett, J. W., 1975, Intercellular communication in the rat anterior pituitary gland: An in vivo and in vitro study, J. Cell Biol. 67: 469.PubMedCrossRefGoogle Scholar
  20. Gilula, N. B., Epstein, M. L., and Beers, W. H., 1978, Cell-to-cell communication and ovulation: A study of the cumulus-oocyte complex, J. Cell Biol. 78: 58.PubMedCrossRefGoogle Scholar
  21. Girod, C., 1977, Apport de l’immunohistochimie à l’étude cytologique de l’adénohypophyse, Bull. Assoc. Anat. 62: 21.Google Scholar
  22. Herbert, D. C., 1975, Localization of antisera to LHβ and FSHβ in the rat pituitary, Am. J. Anat. 144: 379.PubMedCrossRefGoogle Scholar
  23. Herbert, D. C., 1976, Immunocytochemical evidence that luteinizing hormone (LH) and follicle stimulating hormone (FSH) are present in the same cell type in the rhesus monkey pituitary gland, Endocrinology 98: 1554.PubMedCrossRefGoogle Scholar
  24. Herlant, M., 1960, Étude de deux techniques nouvelles destinées à mettre en évidence les différentes catégories cellulaires présentes dans la glande pituitaire, Bull. Microsc. Appl. 10: 37.Google Scholar
  25. Herlant, M., 1964, The cells of the adenohypophysis and their functional significance, Int. Rev. Cytol. 17: 299.PubMedCrossRefGoogle Scholar
  26. Hymer, W. C., 1975, Separation of organelles and cells from the mammalian adenohypophysis, in: The Anterior Pituitary (A. Tixier-Vidal and M. G. Farquhar, eds.), p. 137, Academic Press, New York.Google Scholar
  27. Hymer, W. C., Evans, W. H., Kraicer, J., Mastro, A., Davis, J., and Griswold, E., 1973, Enrichment of cell types from the rat adenohypophysis by sedimentation at unit gravity, Endocrinology 92: 275.PubMedCrossRefGoogle Scholar
  28. Hymer, W. C., Snyder, J., Wilfinger, W., Swanson, N., and Davis, J. A., 1974, Separation of pituitary mammotrophs from the female rat by velocity sedimentation at unit gravity, Endocrinology 95: 107.PubMedCrossRefGoogle Scholar
  29. Kalra, S. P., 1976, Ovarian steroids differentially augment pituitary FSH release in deafferented rats, Brain Res. 114: 541.PubMedCrossRefGoogle Scholar
  30. Kao, L. W. L., and Weisz, J., 1975, Direct effect of testosterone and its 5α-reduced metabolites on pituitary LH and FSH release in vitro: Change in pituitary responsiveness to hypothalamic extract, Endocrinology 96: 253.PubMedCrossRefGoogle Scholar
  31. Kragt, C. L., and Dahlgren, J., 1972, Development of neural regulation of follicle stimulating hormone (FSH) secretion, Neuroendocrinology 9: 30.PubMedCrossRefGoogle Scholar
  32. Kraicer, J., and Hymer, W. C., 1974, Purified somatotrophs from rat adenohypophysis: Response to secretagogues, Endocrinology 94: 1525.PubMedCrossRefGoogle Scholar
  33. Kurosumi, K., and Oota, Y., 1968, Electron microscopy of two types of gonadotrophs in anterior pituitary glands of persistent estrous and diestrous rats, Z. Zeilforsch. 85: 34.CrossRefGoogle Scholar
  34. Lawrence, T. S., Beers, W. H., and Gilula, N. B., 1978, Transmission of hormonal stimulation by cell-to-cell communication, Nature (London) 272: 501.CrossRefGoogle Scholar
  35. Lloyd, R. V., and Karavolas, H. J., 1975, Uptake and conversion of progesterone and testosterone to 5α-reduced products by enriched gonadotropic and chromophobic rat anterior pituitary cell fractions, Endocrinology 97: 517.PubMedCrossRefGoogle Scholar
  36. Lloyd, R. V., and McShan, W. H., 1973, Study of rat anterior pituitary cells separated by velocity sedimentation at unit gravity, Endocrinology 92: 1639.PubMedCrossRefGoogle Scholar
  37. Miller, W. L., Knight, M. M., Grimek, H. J., and Gorski, J., 1977, Estrogen regulation of follicle stimulating hormone in cell cultures of sheep pituitaries, Endocrinology 100: 1306.PubMedCrossRefGoogle Scholar
  38. Mittler, J. C., 1974, Androgen effects on follicle-stimulating hormone (FSH) secretion in organ culture, Neuroendocrinology 16: 265.PubMedCrossRefGoogle Scholar
  39. Moriarty, G. C., 1975, Electron microscopic-immunocytochemical studies of rat pituitary gonadotrophs: A sex difference in morphology and cytochemistry of LH cells, Endocrinology 97: 1215.PubMedCrossRefGoogle Scholar
  40. Moriarty, G. C., 1976, Immunocytochemistry of the pituitary glycoprotein hormones, J. Histochem. Cytochem. 24: 846.PubMedCrossRefGoogle Scholar
  41. Nakane, P. K., 1970, Classification of anterior pituitary cell types with immunoenzyme histochemistry, J. Histochem. Cytochem. 18: 9.PubMedCrossRefGoogle Scholar
  42. Ojeda, S. R., and Ramirez, V. D., 1972, Plasma levels of LH and FSH in maturing rats: Response to hemigonadectomy, Endocrinology 90: 466.PubMedCrossRefGoogle Scholar
  43. Ojeda, S. R., Jameson, H. E., and McCann, S. M., 1977, Developmental changes in pituitary responsiveness to luteinizing hormone-releasing hormone (LHRH) in the female rat: Ovarian adrenal influence during the infantile period, Endocrinology 100: 440.PubMedCrossRefGoogle Scholar
  44. Ondo, J. G., Pass, K. A., and Baldwin, R., 1976, The effects of neurally active amino acids on pituitary gonadotropin secretion, Neuroendocrinology 21: 79.PubMedCrossRefGoogle Scholar
  45. Pelletier, G., Leclerc, R., and Labrie, F., 1976, Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique, Mol. Cell. Endocrinol. 6: 123.PubMedCrossRefGoogle Scholar
  46. Phifer, R. F., Midgley, A. R., and Spicer, S. S., 1973, Immunohistologic and histologic evidence that follicle-stimulating hormone and luteinizing hormone are present in the same cell type in the human pars distalis, J. Clin. Endocrinol. Metab. 36: 125.PubMedCrossRefGoogle Scholar
  47. Robyn, C., Leleux, P., Vanhaelst, L., Golstein, J., Herlant, M., and Pasteels, J. L., 1973, Immunohistochemical study of the human pituitary with anti-luteinizing hormone, anti-follicle stimulating hormone and anti-thyrotrophin sera, Acta Endocrinol 72: 625.PubMedGoogle Scholar
  48. Schally, A. V., Arimura, A., and Kastin, A. J., 1973a, Hypothalamic regulatory hormones, Science 179: 341.PubMedCrossRefGoogle Scholar
  49. Schally, A. V., Redding, T. W., and Arimura, A., 1973b, Effect of sex steroids on pituitary responses to LH-and FSH-releasing hormone in vitro, Endocrinology 93: 893.PubMedCrossRefGoogle Scholar
  50. Schally, A. V., Coy, D. H., and Meyers, C. A., 1978, Hypothalamic regulatory hormones, Annu. Rev. Biochem. 47: 89.PubMedCrossRefGoogle Scholar
  51. Siperstein, E., Nichols, C. W., Griesbach, W. E., and Chaikoff, I. L., 1954, Cytochemical changes in the rat anterior pituitary from birth to maturity, Anat. Rec. 118: 593.PubMedCrossRefGoogle Scholar
  52. Snyder, G., Hymer, W. C., and Snyder, J., 1977, Functional heterogeneity in somatotrophs isolated from the rat anterior pituitary, Endocrinology 101: 788.PubMedCrossRefGoogle Scholar
  53. Snyder, J., Wilfinger, W., and Hymer, W. C., 1976, Maintenance of separated rat pituitary mammotrophs in cell culture, Endocrinology 98: 25.PubMedCrossRefGoogle Scholar
  54. Spona, J., 1976, Action of steroids on LH-RH provoked gonadotrophin release, Endocrinol. Exp. 10: 91.PubMedGoogle Scholar
  55. Sternberger, L. A., and Petrali, J. P., 1975, Quantitative immunocytochemistry of pituitary receptors for luteinizing hormone-releasing hormone, Cell Tissure Res. 162: 141.Google Scholar
  56. Surks, M. I., and De Fesi, Ch. R., 1977, Determination of the cell number of each cell type in the anterior pituitary of euthyroid and hypothyroid rats, Endocrinology 101;946.Google Scholar
  57. Tang, L. K. L., and Spies, H. G., 1975, Effects of gonadal steroids on the basal and LRF-induced gonadotropin secretion by cultures of rat pituitary, Endocrinology 96: 349.PubMedCrossRefGoogle Scholar
  58. Tixier-Vidal, A., Gourdji, D., and Tougard, C., 1975a, A cell culture approach to the study of anterior pituitary cells, Int. Rev. Cytol. 41: 173.PubMedCrossRefGoogle Scholar
  59. Tixier-Vidal, A., Tougard, C., Kerdelhué, B., and Jutisz, M., 1975b, Light and electron microscopic studies on immunocytochemical localization of gonadotropic hormones in the rat pituitary gland with antisera against ovine FSH, LH, LHα, and LHβ, Ann. N. Y. Acad. Sci. 254: 433.PubMedCrossRefGoogle Scholar
  60. Tougard, C., Kerdelhué, B., Tixier-Vidal, A., and Jutisz, M., 1973, Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase-labeled antibody technique, J. Cell Biol. 58: 503.PubMedCrossRefGoogle Scholar
  61. Tougard, C., Picart, R., and Tixier-Vidal, A., 1977a, Cytogenesis of immunoreactive gonadotropic cells in the fetal rat pituitary at light and electron microscope levels, Dev. Biol. 58: 148.PubMedCrossRefGoogle Scholar
  62. Tougard, C., Tixier-Vidal, A., Kerdelhué, B., and Jutisz, M., 1977b, Étude immunocyto-chimique de l’évolution des cellules gonadotropes dans des cultures primaires de cellules antéhypophysaires de rat: Aspects quantitatifs et ultrastructuraux, Biol. Cell. 28: 251.Google Scholar
  63. Yoshimura, F., and Harumiya, K., 1965, Electron microscopy of the anterior lobe of pituitary in normal and castrated rats, Endocrinol. Jpn. 12: 119.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • Carl Denef
    • 1
  1. 1.Laboratory of Cell Pharmacology, Department of Pharmacology, School of Medicine, Campus GasthuisbergKatholieke Universiteit LeuvenLeuvenBelgium

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