The Release of ACTH by Human Pituitary and Tumor Cells in a Superfusion System
Investigators in the field of biochemical endocrinology may find themselves in a difficult position when they face a decision whether to perform in vivo or in vitro experiments and when comparing data from these tests. In pituitary hormone studes, there always has been and there always will be the dilemma of either (1) leaving the pituitary in situ, where one does not know exactly which of many possible factors could affect pituitary function but where one is certain of the “physiology” of the experiment, or (2) removing the gland to an in vitro setup, where the experimenter decides exactly which substances or factors he wants pituitary function to be affected by, but where there will always be doubts about how closely the experimental conditions approach “normal physiology.” In view of the many difficulties encountered in setting up well-controlled in vivo experiments or in the interpretation of their results, we thought it desirable to perform in vitro experiments accompanied by as many in vivo parallels as possible and feasible. We have therefore devoted some time recently to the development of in vitro experimental procedures that we think approximate the in vivo situation to a substantial degree.
KeywordsPituitary Cell ACTH Secretion Pituitary Tissue ACTH Release Pituitary Tumor Cell
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