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Pregrowth Hormone Messenger RNA: Glucocorticoid Induction and Purification from Rat Pituitary Cells

  • F. Carter Bancroft
  • Paul R. Dobner
  • Li-Yuan Yu
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

For several years, our laboratory has been engaged in studies of the biosynthesis of growth hormone by the rat pituitary. These studies have had two goals, which are clearly interdependent. The first goal has been the elucidation of the steps involved in the synthesis of growth hormone. Thus, we hope ultimately to be able to provide a complete description at the molecular level of the structure and transcription of the growth hormone gene, the structure of the resultant RNA transcript, the processing of this transcript into mature cytoplasmic growth hormone messenger RNA (mRNA), the structure of the primary protein product of the translation of this mRNA, and the processing of this primary translation product into mature growth hormone. The second goal has involved an understanding of the hormonal regulation of the expression of the growth hormone gene. Studies with a number of model systems have suggested that steroid hormones regulate specific gene expression at a pretransla-tional level, via a general mechanism that involves localization in the nucleus of steroid bound to a specific cytoplasmic receptor (reviewed in Yamamoto and Alberts, 1976). We showed some years ago that glucocor-ticoid hormones such as hydrocortisone stimulate the production of growth hormone by cultured rat pituitary cells (Bancroft et al., 1969). Our second goal has thus been to determine both the step(s) in the production of growth hormone at which glucocorticoid hormones act and the molecular mechanisms whereby the stimulation by glucocorticoids is exerted.

Keywords

Growth Hormone Growth Hormone Gene Growth Hormone mRNA mRNA Activity Wheat Germ Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aviv, H., and Leder, P., 1972, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose, Proc. Nail. Acad. Sci. U.S.A. 69: 1408.CrossRefGoogle Scholar
  2. Bancroft, F. C., 1973, Intracellular location of newly synthesized growth hormone, Exp. Cell Res. 79: 275.PubMedCrossRefGoogle Scholar
  3. Bancroft, F. C., Levine, L., and Tashjian, A. H., Jr., 1969, Control of growth hormone production by a clonal strain of rat pituitary cells: Stimulation by hydrocortisone, J. Cell Biol. 43: 432.PubMedCrossRefGoogle Scholar
  4. Bancroft, F. C., Wu, G.-J., and Zubay, G., 1973, Cell-free synthesis of rat growth hormone, Proc. Natl. Acad. Sci. U.S.A. 70: 3646.PubMedCrossRefGoogle Scholar
  5. Evans, G. A., David, D. N., and Rosenfeld, M. G., 1978, Regulation of prolactin and somatotropin mRNAs by thyroliberin, Proc. Natl. Acad. Sci. U.S.A. 75: 1294.PubMedCrossRefGoogle Scholar
  6. Harpold, M. M., Dobner, P. R., Evans, R. M., and Bancroft, F. C., 1978, Construction and identification by positive hybridization-translation of a bacterial plasmid containing a rat growth hormone structural sequence, Nucleic Acids Res. 5: 2039.PubMedCrossRefGoogle Scholar
  7. Kamine, J., and Buchanan, J. M., 1977, Cell-free synthesis of two proteins unique to RNA of transforming virions of Rous sarcoma virus, Proc. Natl. Acad. Sci. U.S.A. 74: 2011.PubMedCrossRefGoogle Scholar
  8. Kessler, S. W., 1975, Rapid isolation of antigens from cells with a Staphylococcal protein A-antibody absorbent: Parameters of the interaction of antibody-antigen complexes with protein A, J. Immunol. 115: 1617.PubMedGoogle Scholar
  9. Longacre, S. S., and Rutter, W. J., 1977, Isolation of chicken hemoglobin mRNA and synthesis of complementary DNA, J. Biol. Chem. 252: 2742.PubMedGoogle Scholar
  10. Martial, J. A., Seeburg, P. H., Guenzi, D., Goodman, H. M., and Baxter, J. D., 1977, Regulation of growth hormone gene expression: Synergistic effects of thyroid and glucocorticoid hormones, Proc. Natl. Acad. Sci. U.S.A. 74: 4293.PubMedCrossRefGoogle Scholar
  11. Martin, T. F. J., and Tashjian, A. H., Jr., 1977, Cell culture studies of thyrotropin-releasing hormone action, in: Biochemical Actions of Hormones, Vol. IV (G. Litwack, ed.), pp. 269–312, Academic Press, New York.CrossRefGoogle Scholar
  12. Palmiter, R. D., 1974, Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded poly somes and messenger ribonucleic acid, Biochemistry 13: 3606.PubMedCrossRefGoogle Scholar
  13. Pelham, H. R. B., and Jackson, R. J., 1976, An efficient mRNA-dependent translation system from reticulocyte lysates, Eur. J. Biochem. 67: 247.PubMedCrossRefGoogle Scholar
  14. Schimke, R. T., Rhoads, R. E., and McKnight, G. S., 1974, Assay of ovalbumin mRNA in reticulocyte lysates, in: Methods in Enzymology, Vol. 30F (L. Grossman and K. Moldave, eds.), pp. 694–701, Academic Press, New York.Google Scholar
  15. Shapiro, L. E., Samuels, H. H. and Yaffe, B. M., 1978, Thyroid and glucocorticoid hormones synergistically control growth hormone mRNA in cultured GH1 cells, Proc. Natl. Acad. Sci. U.S.A. 75: 45.PubMedCrossRefGoogle Scholar
  16. Spielman, L. L., and Bancroft, F. C., 1977, Pregrowth hormone: Evidence for conversion to growth hormone during synthesis on membrane bound polysomes, Endocrinology 101: 651.PubMedCrossRefGoogle Scholar
  17. Sussman, P. M., Iushinski, R. J., and Bancroft, F. C., 1976, Pregrowth hormone: Product of the translation in vitro of messenger RNA coding for growth hormone, Proc. Natl. Acad. Sci. U.S.A. 73: 29.PubMedCrossRefGoogle Scholar
  18. Sussman-Berger, P. M., 1978, Discovery and characterization of the precursor of rat growth hormone, Ph.D. thesis, Department of Biological Sciences, Columbia University, New York.Google Scholar
  19. Tashjian, A. H., Jr., Bancroft, F. C., and Levine, L., 1970, Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells: Differential effects of hydrocortisone and tissue extracts, J. Cell Biol. 47: 61.PubMedCrossRefGoogle Scholar
  20. Tushinski, R. J., Sussman, P. M., Yu, L.-Y., and Bancroft, F. C., 1977, Pregrowth hormone messenger RNA: Glucocorticoid induction and identification in rat pituitary cells, Proc. Natl. Acad. Sci. U.S.A. 74: 2357.PubMedCrossRefGoogle Scholar
  21. Yamamoto, K. R., and Alberts, B. M., 1976, Steroid receptors: Elements for modulation of eucaryotic transcription, Annu. Rev. Biochem. 45: 721.PubMedCrossRefGoogle Scholar
  22. Yu, L.-Y., Tushinski, R. J., and Bancroft, F. C., 1977, Glucocorticoid induction of growth hormone synthesis in a strain of rat pituitary cells, J. Biol. Chem. 252: 3870.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • F. Carter Bancroft
    • 1
  • Paul R. Dobner
    • 1
  • Li-Yuan Yu
    • 1
  1. 1.Cellular Gene Expression and Regulation LaboratoryMemorial Sloan-Kettering Cancer CenterNew YorkUSA

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