Effect of TRH on the Synthesis and Stability of Cytoplasmic RNAs of GH3B6 Cells

  • A. Morin
Part of the Biochemical Endocrinology book series (BIOEND)


Rat prolactin continuous cell lines offer invaluable models for studying the mechanism of action of thyroliberin (TRH) on prolactin (PRL) secretion in a homogeneous population of target cells (Tashjian and Hoyt, 1972; Tixier-Vidal et al., 1975). One of these cell lines, GH3B6, is a subclone of the GH3 strain derived from a transplantable pituitary tumor induced by chronic estrogen treatment (Tashjian et al., 1968). As reported by Tashjian et al. (1970, 1971) and Gourdji et al. (1972) for GH3 cells, GH3B6 cells secrete both PRL and, at a low level, growth hormone (GH), and they respond to TRH by an increase of PRL secretion.


Growth Hormone Cytoplasmic RNAs Chase Experiment Prolactin mRNA Cytoplasmic Poly 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Biswas, D, K., and Tashjian, A. H., Jr., 1974, Intracellular site of prolactin synthesis in rat pituitary cells in culture, Biochem. Biophys. Res. Commun. 60: 241.PubMedCrossRefGoogle Scholar
  2. Buckingham, M. E., Caput, D., Cohen, A., Whalen, R. G., and Gros, F., 1974, The synthesis and stability of cytoplasmic messenger RNA during myoblast differentiation in culture, Proc. Nad. Acad. Sci. U.S.A. 71: 1466.CrossRefGoogle Scholar
  3. Evans, G. A., and Rosenfeld, M. G., 1976, Cell-free synthesis of a prolactin precursor directed by mRNA from cultured rat pituitary cells, J. Biol. Chem. 251: 2842.PubMedGoogle Scholar
  4. Evans, G. A., Hucko, J., and Rosenfeld, M. G., 1977, Preprolactin represents the initial product of prolactin mRNA translation, Endocrinology 101: 1807.PubMedCrossRefGoogle Scholar
  5. Evans, G. A., David, D. N., and Rosenfeld, M. G., 1978, Regulation of prolactin and somatotropin mRNAs by thyroliberin, Proc. Natl. Acad. Sci. U.S.A. 75: 1294.PubMedCrossRefGoogle Scholar
  6. Gourdji, D., Kerdelhué, B., and Tixier-Vidal, A., 1972, Ultrastructure d’un clone de cellules hypophysaires sécrétant de la prolactine (GH3): Modifications induites par l’hormone hypothalamique de libération de l’hormone thyréotrope (TRF), C. R. Acad. Sci. Ser. D 274: 437.Google Scholar
  7. Martin, T. F. J., and Tashjian A. H., Jr., 1978, Thyrotropin-releasing hormone modulation of uridine uptake in rat pituitary cells: Evidence that uridine phosphorylation is regulated, J. Biol. Chem. 253: 10PubMedGoogle Scholar
  8. Morin, A., Tixier-Vidal, A., Gourdji, D., Kerdelhué, B., and Grouselle, D., 1975, Effect of thyrotropin-releasing hormone (TRH) on prolactin turnover in culture, Mol. Cell. Endocrinol. 3: 351.PubMedCrossRefGoogle Scholar
  9. Sheldon, R., Jurale, C., and Kates, J., 1972, Detection of polyadenylic acid sequences in viral and eukaryotic RNA, Proc. Nail. Acad. Sci. U.S.A. 69: 417.CrossRefGoogle Scholar
  10. Tashjian, A. H., Jr., and Hoyt, R. F., Jr., 1972, Transient controls of organ specific functions in pituitary cells in culture, in: Molecular Genetics and Developmental Biology (M. Sussman, ed.), pp. 353–387, Prentice-Hall, Englewoods Cliffs, New Jersey.Google Scholar
  11. Tashjian A. H., Jr., Yasumura, Y., Levine, L., Sato, G. H., and Parker, M. L., 1968, Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone, Endocrinology 82: 342.PubMedCrossRefGoogle Scholar
  12. Tashjian A. H., Jr., Bancroft, F. C., and Levine, L., 1970, Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells: Differential effects of hydrocortisone and tissue extracts, J. Cell Biol. 47: 61.PubMedCrossRefGoogle Scholar
  13. Tashjian A. H., Jr., Barowsky, N. J., and Jensen, D. K., 1971, Thyrotropin releasing hormone: Direct evidence for stimulation of prolactin production by pituitary cells in culture, Biochem. Biophys. Res. Commun. 43: 516.PubMedCrossRefGoogle Scholar
  14. Tixier-Vidal, A., Gourdji, D., and Tougard, C., 1975, A cell culture approach to the study of anterior pituitary cells, Int. Rev. Cytol. 41: 173.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • A. Morin
    • 1
  1. 1.Groupe de Neuroendocrinologie Cellulaire, Laboratoire de Physiologie CellulaireCollège de FranceParis 05France

Personalised recommendations