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Toward Development of Assays for Scrapie-Specific Antibodies

  • Kenneth C. Kasper
  • Karen Bowman
  • Daniel P. Stites
  • Stanley B. Prusiner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 134)

Abstract

At least 40 attempts have been made to detect antibodies directed against the scrapie agent (Table 1). All have failed. More than 10 were by neutralization (1–6), seven by precipitation in gel or tubes (3,7–9), eight by complement fixation (3,5,7), and six by direct or indirect immunofluorescence procedures (3,6,8). Passive hemagglutination, passive hemolysis, passive anaphylaxis, immunoconglutinin (7), and anti-nuclear (10) and anti-cardiolipin (7) antibody tests were also employed. Antisera from goats, sheep, mice, rabbits, roosters, monkeys and humans have been tested.

Keywords

Bovine Serum Albumin Vesicular Stomatitis Virus Scrapie Agent Hamster Brain Goat Brain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Kenneth C. Kasper
    • 1
  • Karen Bowman
    • 1
  • Daniel P. Stites
    • 1
  • Stanley B. Prusiner
    • 1
  1. 1.Howard Hughes Medical Institute Laboratory, Departments of Neurology, Biochemistry and Biophysics, Laboratory Medicine and MedicineUniversity of California, School of MedicineSan FranciscoUSA

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