Hamster Lymphoid Cell Responses in Vitro
Lymphoid cells localized to different areas within an animal appear to perform specific immune functions. Their reactivity in vivo is dependent on the microenvironment within a particular tissue as well as the ability of a tissue to attract and accumulate specific re-circulating elements. While in vitro culturing of lymphoid cells does not accurately approximate the in vivo situation, information regarding differences among lymphoid tissues in responsiveness to a variety of specific and non-specific stimulants can be useful in assessing the potential of different tissues. That is, the responsiveness of lymphoid tissues (lymph node, spleen, thymus, bone marrow, peripheral blood) to non-specific mitogens such as concanavalin A (Con A), phytohemagglutinin (PHA), lipopolysaccharide (LPS), dextran sulfate (DxS), proteases, anti-immunoglobulin (anti-Ig) or mitogenic ions (Zn+2 and Hg+2) can yield information concerning the compartmentalization of subpopulations of lymphocytes or accessory cells. Similarly, characterization of antigen-driven responses in vitro can yield information concerning compartmentalization as well as information on the regulation of activation and differentiation of lymphoid cells which may differ from those employed for mitogen activation. In vitro culture of lymphoid cells also permits evaluation of agents that can modulate the activity of lymphoid cells.
KeywordsLymphoid Cell Lymph Node Cell Proteolytic Event Spleen Cell Culture States Public Health Service Grant
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