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Hamster Lymphoid Cell Responses in Vitro

  • David A. Hart
  • Joan Stein-Streilein
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 134)

Abstract

Lymphoid cells localized to different areas within an animal appear to perform specific immune functions. Their reactivity in vivo is dependent on the microenvironment within a particular tissue as well as the ability of a tissue to attract and accumulate specific re-circulating elements. While in vitro culturing of lymphoid cells does not accurately approximate the in vivo situation, information regarding differences among lymphoid tissues in responsiveness to a variety of specific and non-specific stimulants can be useful in assessing the potential of different tissues. That is, the responsiveness of lymphoid tissues (lymph node, spleen, thymus, bone marrow, peripheral blood) to non-specific mitogens such as concanavalin A (Con A), phytohemagglutinin (PHA), lipopolysaccharide (LPS), dextran sulfate (DxS), proteases, anti-immunoglobulin (anti-Ig) or mitogenic ions (Zn+2 and Hg+2) can yield information concerning the compartmentalization of subpopulations of lymphocytes or accessory cells. Similarly, characterization of antigen-driven responses in vitro can yield information concerning compartmentalization as well as information on the regulation of activation and differentiation of lymphoid cells which may differ from those employed for mitogen activation. In vitro culture of lymphoid cells also permits evaluation of agents that can modulate the activity of lymphoid cells.

Keywords

Lymphoid Cell Lymph Node Cell Proteolytic Event Spleen Cell Culture States Public Health Service Grant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • David A. Hart
    • 1
  • Joan Stein-Streilein
    • 1
  1. 1.Department of MicrobiologyThe University of Texas Health Science Center at DallasDallasUSA

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