Isolation and Characterization of Hamster Alpha2 Macroglobulin

  • Joan Stein-Streilein
  • David A. Hart
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 134)


Previous reports from our laboratory have described conditions for culturing hamster lymphocytes with mitogens in the absence of serum (1). This finding allowed us to investigate the possibility that a humoral factor or serum components may affect the in vitro activity of different cell populations. We have reported that a specific inhibitor of lipopolysaccharide (LPS) stimulation of MHA spleen and lymph node cells and of dextran sulfate (DxS) stimulation of spleen cells is present in normal isologous serum (1). This component does not affect Con A stimulation or mixed lymphocyte reactivity and, therefore, appears to be specific for a particular subpopulation of lymphoid cells (B lymphocytes). We now report that the high molecular weight inhibitor of serum or plasma is alpha2 macroglobulin (α2M).


Dextran Sulfate Mixed Lymphocyte Reactivity Serum Fraction Alpha2 Macroglobulin Alpha Globulin 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Joan Stein-Streilein
    • 1
  • David A. Hart
    • 1
  1. 1.Department of MicrobiologyUniversity of Texas Health Science Center at DallasDallasUSA

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