Methods for Bioassays of Tobacco Smoke

  • F. G. Bock
Conference paper


Cigarette smoking has been associated with a number of diseases in man. In most cases this relationship has required a. long period of exposure and the incidence of disease is dependent upon the number of cigarettes consumed. Attempts to interrupt a sequence between smoking and disease have presented us with a. series of very complex problems, most of which have not yet been solved. First of all it is unlikely that any single constituent of cigarette smoke could account for all of the conditions which develop in a group of heavy cigarette smokers. Many separate assay procedures will be required to determine effects of cigarette design on the delivery of potentially hazardous agents in the smoke, and these procedures are not available today. I think it is fair to say that although no fully satisfying laboratory model of the various diseases is available, the models for carcinogenesis offer promise that something positive can be achieved in this area.


Tobacco Smoke Tumor Promoter Mouse Skin Skin Painting Smoking Product 
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  1. 1.
    Pott, P. Chirurgical Observations Relative to the Cataract, the Polypus of the Nose, the Cancer of the Scrotum, the Different Kinds of Ruptures and the Mortification of the Toes and Feet. London, Hawes, Clarke, & Collins, 1775.Google Scholar
  2. 2.
    Yamagiwa, K. and Ichikawa, K. Experimental Study of the Pathogenesis of Carcinoma. J. Cancer Res. 3:1–21, 1918.Google Scholar
  3. 3.
    Wynder, E. L. and Hoffmann D. Tobacco and Tobacco Smoke. Studies in Experimental Carcinogenesis, New York, Academic Press, Inc., 1967.Google Scholar
  4. 4.
    Berenblum, I. A Re-evaluation of the Concept of Cocarcino-genesis. Progr. Exper. Tumor Res. 11:21–30, 1969.Google Scholar
  5. 5.
    Berenblum, I. The Mechanism of Carcinogenesis: A Study of the Significance of Cocarcinogenic Action and Related Phenomena. Cancer Res. 1:807–814, 1941.Google Scholar
  6. 6.
    Berenblum, I. and Shubik, P. A New Quantitative Approach to the Study of Stages of Chemical Carcinogenesis in the Mouse’s Skin. Brit. J. Cancer 1:383–391, 1947.CrossRefGoogle Scholar
  7. 7.
    Saffiotti, U. and Shubik, P. The Effects of Low Concentrations of Carcinogen in Epidermal Carcinogenesis. A Comparison with Promoting Agents. J. Nat. Cancer Inst. 16:961–969, 1956.Google Scholar
  8. 8.
    Boutwell, R. K. Some Biological Aspects of Skin Carcinogenesis. Progr. exp. Tumor Res. 4:207–250, 1964.Google Scholar
  9. 9.
    Eastcott, D. F. The Epidemiology of Lung Cancer in New Zealand. Lancet 1:37–39, 1956.CrossRefGoogle Scholar
  10. 10.
    Dean, G. Lung Cancer Among White South Africans. Brit. Med. J. 2:852–859, 1959.CrossRefGoogle Scholar
  11. 11.
    Doll, R. Interpretations of Epidemiologic Data. Cancer Res. 23:1613–1623, 1963.Google Scholar
  12. 12.
    Selikoff, I. J., Hammond, E. C., and Churg, J. Asbestos Exposure, Smoking, and Neoplasia. J. Am. Med. Assoc. 204:106–112, 1968.CrossRefGoogle Scholar
  13. 13.
    Bair, W. J. Inhalation of Radionuclides and Carcinogenesis. In: Inhalation Carcinogenesis, ed. M. G. Hanna Jr., P. Nettesheim, and J. R. Gilbert, pp„ 77–101, USAEC Div. of Tech. Inform., Oak Ridge, Tenn., 1970.Google Scholar
  14. 14.
    Bock, F. G. The Nature of Tumor-promoting Agents in Tobacco Products. Cancer Res. 28:2363–2368, 1968.Google Scholar
  15. 15.
    Baird, W. M. and Boutwell R. K. Tumor-promoting Activity of Phorbol and Four Diesters of Phorbol in Mouse Skin. Cancer Res. 31:1074–1079, 1971.Google Scholar
  16. 16.
    Holsti, P. Tumor Promoting Effects of Some Long Chain Fatty Acids in Experimental Skin Carcinogenesis in the Mouse. Acta Path. microbiol. Scand. 46:51–58, 1959.CrossRefGoogle Scholar
  17. 17.
    Setälä, K., Setälä, H., Merenmies, L. and Holsti P. Untersuchunger über die tumorauslosende (“tumour promoting”) Wirkung einiger nichtionisierbaren oberflächenaktiven Substanzen bei Maus und Kaninchen. Z. Krebsforsch. 61: 534–547, 1957.CrossRefGoogle Scholar
  18. 18.
    Muñoz, N., Correa, P., and Bock, F. G. Comparative Carcinogenic Effect of Tow Types of Tobacco. Cancer 21:376–389, 1968.CrossRefGoogle Scholar
  19. 19.
    Borneff, J., and Fischer, R. Kanzerogene Substanzen in Wasser und Boden XI. Polyzyklische, aromatische Kohlenwasserstoffe in Walderde. Arch. Hyg. Bacteriol. 146:430–437, 1962.Google Scholar
  20. 20.
    Harington, J. S. Chemical Studies of Asbestos. Ann. N. Y. Acad. Sci. 132:31–47, 1965.CrossRefGoogle Scholar
  21. 21.
    Sawicki, E., Hauser, T. R., Elbert, W., Fox, F. T., and Meeker, J. E. Polynuclear Aromatic Hydrocarbon Composition of the Atmosphere in Seven Large American Cities. J. Am. Ind. Hyg. Assoc. 23:137–143, 1962.CrossRefGoogle Scholar
  22. 22.
    Hoffmann, D., and Wynder, E. L. A Study of Tobacco Carcinogenesis XI. Tumor Initiators, Tumor Accelerators, and Tumor Promoting Activity of Condensate Fractions. Cancer 27: 848–864, 1971.CrossRefGoogle Scholar
  23. 23.
    Bock, F. G. Dose Response: Experimental Carcinogenesis. Nat. Cancer Inst. Mono. 28:57–63, 1968.Google Scholar
  24. 24.
    Bock, F. G. Early Effects of Hydrocarbons on Mammalian Skin. Progr. exp. Tumor Res. 4:126–168, 1964.Google Scholar
  25. 25.
    Shimkin, M. B. and Lorenz, E. Factors Influencing the Induction of Pulmonary Tumors in Strain A Mice by Carcinogenic Hydrocarbons. J. Nat. Cancer Inst. 2:499–510, 1942.Google Scholar
  26. 26.
    Rockey, E. E. and Speer, F. D. The Ill Effects of Cigarette Smoking in Dogs. Intern. Surg. 46:520–530, 1966.Google Scholar
  27. 27.
    Dontenwill, W. Experimental Investigations on the Effect of Cigarette Smoke Inhalation on Small Laboratory Animals. In: Inhalation Carcinogenesis, ed. M. G. Hanna Jr., P. Nettesheim, and J. R. Gilbert, pp. 389–412, USAEC Div. of Tech. Inform., Oak Ridge, Tenn., 1970.Google Scholar
  28. 28.
    Auerbach, Oo, Hammond, E. C., Kirman, D. and Garfinkel, L. Effects of Cigarette Smoking on Dogs. II. Pulmonary Neoplasms. Arch. of Env. Health 21:754–768, 1970.Google Scholar
  29. 29.
    Chamberlain, Wo J. and Stedman, R. L. Fractionation of Tobacco Smoke Condensate for Chemical Composition Studies. This monograph.Google Scholar
  30. 30.
    Bock, F. G., Swain, A. P., and Stedman, R. L. Composition Studies on Tobacco. XLIV. Tumor-Promoting Activity of Sub-fractions of the Weak Acid Fraction of Cigarette Smoke Condensate. J. Nat. Cancer Inst. 47:429–436, 1971.Google Scholar
  31. 31.
    Bock, F. G., Swain, A. P., and Stedman, R. L. Unpublished.Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • F. G. Bock
    • 1
  1. 1.Roswell Park Memorial InstituteNew York State Department of HealthBuffaloUSA

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