Assembly of Mouse Hepatitis Virus Strain JHM
Coronaviruses have been characterized as a separate group (26), predominantly based on the morphology of their unique, massive peplomers and single stranded +RNA genome (22). Electron microscopic studies on different members of this group revealed that assembly occurs in the cytoplasm, where progeny are formed by a budding process from membranes of either the endoplasmic reticulum and/or cytoplasmic vacuoles (9). The budding process has been described in some detail in the case of avian infectious bronchitis virus, the human agent, 229E (2), and other isolates (3, 7, 10, 16, 18, 23, 24, 25, 29, 30). Despite the preponderance of data favouring budding as the assembly mechanism some reservations have been made about the significance of this process (11, 27). The present electron microscopic study was undertaken to ascertain at high resolution the nature of coronavirus assembly, with particular attention to the incompletely characterized nucleocapsid.
KeywordsInfluenza Virus Infectious Bronchitis Virus Cytoplasmic Vacuole Internal Component Chicken Embryo Fibroblast
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