Characterisation of Viral RNA in Cells Infected with the Murine Coronavirus JHM

  • Helmut Wege
  • Stuart Siddell
  • Margarete Sturm
  • Volker ter Meulen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)

Summary

The murine coronavirus JHM induces in Sac(-) cells seven major and two minor RNA species. These RNAs are polyadenylated and single stranded. Their sizes were estimated by electrophoresis in agarose gels containing methyl-mercury hydroxide. The mol. wts. for the major species are 6.67 × 106 for RNA of genome size, 3.42 × 106 for RNA 2, 2.76 × 106 for RNA 3, 1.35 × 106 for RNA 4, 1.19 × 106 for RNA 5, 0.93 × 106 for RNA 6 and 0.62 × 106 for RNA 7. The minor species have a size of 4.7 × 10 (RNA a) and 1.5 x 10 (RNA b). No essential difference in the number and proportion of each RNA species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes, nor was any difference found during the infection cycle. The major RNA species are likely to be subgenomic mRNAs.

Keywords

Sucrose Mercury DMSO Hydroxide Amide 

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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Helmut Wege
    • 1
  • Stuart Siddell
    • 1
  • Margarete Sturm
    • 1
  • Volker ter Meulen
    • 1
  1. 1.Institute for Virology and ImmunobiologyUniversity of WürzburgFederal Republic of Germany

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