Characterisation of Viral RNA in Cells Infected with the Murine Coronavirus JHM

  • Helmut Wege
  • Stuart Siddell
  • Margarete Sturm
  • Volker ter Meulen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


The murine coronavirus JHM induces in Sac(-) cells seven major and two minor RNA species. These RNAs are polyadenylated and single stranded. Their sizes were estimated by electrophoresis in agarose gels containing methyl-mercury hydroxide. The mol. wts. for the major species are 6.67 × 106 for RNA of genome size, 3.42 × 106 for RNA 2, 2.76 × 106 for RNA 3, 1.35 × 106 for RNA 4, 1.19 × 106 for RNA 5, 0.93 × 106 for RNA 6 and 0.62 × 106 for RNA 7. The minor species have a size of 4.7 × 10 (RNA a) and 1.5 x 10 (RNA b). No essential difference in the number and proportion of each RNA species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes, nor was any difference found during the infection cycle. The major RNA species are likely to be subgenomic mRNAs.


Mouse Hepatitis Virus General Virology Uridine Incorporation Marker RNAs Subgenomic mRNAs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bailey, J.M. and Davidson, N. (1976). Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70, 75–85.PubMedCrossRefGoogle Scholar
  2. Bond, C.W., Leibowitz, J.L. and Robb, J.A. (1979). Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronavirus JHMV and A59V. Virology 94, 371–384.PubMedCrossRefGoogle Scholar
  3. Floyd, R.W., Stone, M.P. and Joklik, W.K. (1974). Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Analytical Biochemistry 59, 599–609.PubMedCrossRefGoogle Scholar
  4. Lai, M.M.C. and Stohlman, S.A. (1978). RNA of Mouse hepatitis virus. Journal of Virology 26, 236–242.PubMedGoogle Scholar
  5. Loening, U.E. (1968). Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38, 355–365.PubMedCrossRefGoogle Scholar
  6. Lucas, A., Coulter, M., Anderson, R., Dales, S. and Flintoff, W. (1978). In vivo and in vitro models of demyelinating diseases. II. Persistence and host regulated thermosensitivity in cells of neuronal derivation. Virology 88, 325–337.PubMedCrossRefGoogle Scholar
  7. McMaster, G.K. and Carmichael, G.G. (1977). Analysis of single and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Science, U.S.A. 74, 4835–4838.CrossRefGoogle Scholar
  8. Nagashima, K., Wege, H., Meyermann, R. and ter Meulen, V. (1979). Demyelinating encephalomyelitis induced by a long-term corona virus infection in rats. Acta Neuropathologica 45, 205–213.PubMedCrossRefGoogle Scholar
  9. Repik, P. and Bishop, D.H.L. Determination of the molecular weight of animal RNA viral genomes by nuclease digestion. I. Vesicular stomatitis virus and its defective T particle. Journal of Virology 12, 969–983.Google Scholar
  10. Robb, J.A. and Bond, C.W. (1979). Coronaviridae. In: H. Fraenkel-Conrat and R.R. Wagner (ed.), Comprehensive Virology 14, 193–247. Plenum Press, New York.CrossRefGoogle Scholar
  11. Stohlman, S.A., Sakaguchi, A.Y. and Weiner, L.P. (1979) Characterisation of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion. Virology 98, 448–445.PubMedCrossRefGoogle Scholar
  12. Siddell, S., Wege, H., Barthel, A. and ter Meulen, V. (1981). Coronavirus JHM: Intracellular protein synthesis. Journal of General Virology, in press.Google Scholar
  13. Siddell, S., Wege, H., Barthel A. and ter Meulen, V. (1980). Coronavirus JHM: Cell-free synthesis of structural protein p 60. Journal of Virology 33, No. 1, 10–17.PubMedGoogle Scholar
  14. Stanley, W.M. and Bock, R.M. (1965). Isolation and physical properties of the ribosomal ribonucleic acid of E. coli. Biochemistry 7, 1302–1311.CrossRefGoogle Scholar
  15. Wege, H., Wege, Hanna, Nagashima, K. and ter Meulen, V. (1979). Structural polypeptides of the murine coronavirus JHM. Journal of General Virology 42, 37–47.PubMedCrossRefGoogle Scholar
  16. Wege, H., Müller, A. and ter Meulen, V. (1978). Genomic RNA of the murine coronavirus JHM. Journal of General Virology, 41, 217–227.PubMedCrossRefGoogle Scholar
  17. Weiland, E.M., Mussgay F. and Weiland, F. (1978). Nonproducer malignant tumor cells with rescuable sarcoma virus genome isolated from a recurrent Moloney sarcoma. Journal of Experimental Medicine 148, 408–423.PubMedCrossRefGoogle Scholar
  18. Weiner, L.P. (1973). Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus). Archives of Neurology, 28, 293–303.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Helmut Wege
    • 1
  • Stuart Siddell
    • 1
  • Margarete Sturm
    • 1
  • Volker ter Meulen
    • 1
  1. 1.Institute for Virology and ImmunobiologyUniversity of WürzburgFederal Republic of Germany

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