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Genome Structure of Mouse Hepatitis Virus: Comparative Analysis by Oligonucleotide Mapping

  • Michael M. C. Lai
  • Stephen A. Stohlman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)

Abstract

Several natural variants of mouse hepatitis viruses have been compared by the T1-oligonucleotide fingerprinting technique. In general, they have aiverged quite extensively. However, MHV-3, a hepatotropic strain, and A59, a nonpathogenic strain, were found to be extremely related. Yet, each of them contains 2–4 specific oligonucleotides. One of the MHV-3-specific oligonucleotides was mapped in 30S poly(A)-containing RNA or 6–7 Kb from the 3-end and the other near the 5′-end of the genome. These two genetic regions might be associated with viral pathogenicity. In addition, two JHM plaque variants, DL producing large plaques and DS producing small plaques, were also compared. They share almost all T1-oligonucleotides, but each contains one unique spot. The DL-specific oligonucleotide was mapped in 21S poly(A)-containing RNA or at about 4 kb from the 3′-end. Finally, the MHV genome was found to contain the “cap” structure, confirming that it is a positive-stranded RNA.

Keywords

Infectious Bronchitis Virus Rous Sarcoma Virus Nonpathogenic Strain Mouse Hepatitis Virus Specific Spot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Michael M. C. Lai
    • 1
  • Stephen A. Stohlman
    • 1
  1. 1.University of Southern California School of MedicineLos AngelesUSA

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