Characteristics of a Long Term in Vitro Persistent Infection with Human Coronavirus 229E

  • G. Chaloner-Larsson
  • C. M. Johnson-Lussenburg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


In October of 1978, we were successful in establishing an in vitro persistent infection of a human cell line (L132) with human coronavirus, strain 229E (229E). These persistently infected cells have been shedding virus since that time (over 300 cell passages) to the same high titer of 105-106pfu/ml every 48 hours. We have been concerned with studies on both the virus isolated from the cultures (termed VH) and the persistently infected cell cultures per se (termed L132/229E or simply HV). Our preliminary findings have been described at previous meetings and a report has been submitted for publication. A brief summary of these results will be given here to provide the necessary background to our present work.


Infected Cell L132 Cell Virus Yield Infected Culture Persistent Cell 
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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • G. Chaloner-Larsson
    • 1
  • C. M. Johnson-Lussenburg
    • 1
  1. 1.Department of Microbiology and Immunology School of MedicineUniversity of OttawaOttawa, OntarioCanada

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