Persistent Infection with Mouse Hepatitis Virus, JHM Strain in DBT Cell Culture
After inoculation with JHM strain into DBT cell monolayers, a persistently infected DBT cell culture was established without producing typical cytopathic changes after about 15th passages. By im-munofluorescence virus specific antigen was demonstrated in 10 to 15% DBT cells. This persistently infected culture (JHM-CC) was resistant to superinfection with parental JHM, but such resistance was not shown against vesicular stomatitis virus. JHM-CC virus produced small plaques on DBT cell monolayers. Temperature sensitive (TS) mutant, defective interfering (DI) particle or interferon was not detected in the JHM-CC. To intracerebral inoculation with JHM-CC virus, cortisone treated ICR mice survived without showing clinical signs, however, demyelinating lesions were produced in the brain and spinal cord of them.
KeywordsPersistent Infection Vesicular Stomatitis Virus Canine Distemper Virus Mouse Hepatitis Virus Small Plaque
Unable to display preview. Download preview PDF.
- Fujiwara, K., 1969, Problems in checking inapparent infections in laboratory mouse colonies. An attempt at serological checking by anamnestic response. In: Difining of the laboratory animals. Nat. Acad. Sci. Wash., D.C. 77–97.Google Scholar
- Hirano, N., Murakami, K., Fujiwara, K., and Matumoto, M., 1978, Utility of mouse cell line DBT for propagation and assay of mouse hepatitis virus. Japan. J. Exp. Med. 48: 71–75.Google Scholar
- Robb, J.A., and Bond, C.W., 1978, Coronaviridae. Comprehensive Virology 14Google Scholar
- Taguchi, F., Hirano, N., Kiuchi, Y., and Fujiwara, K., 1976, Difference in response to mouse hepatitis virus among susceptible mouse strain. Japan. J. Microbiol. 20: 293–302.Google Scholar