Evolution of a Coronavirus during Persistent Infection inVitro

  • Kathryn V. Holmes
  • James N. Behnke
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


Coronaviruses usually infect epithelial cells of the gastro-enteric or respiratory tracts. They frequently cause local infections which may be fatal in young animals, but which are usually mild or asymptomatic in adults. In vitro, coronaviruses may induce virulent, cytolytic and cell fusing effects in some host cells but cause a moderate, persistent infection in other cell types. Thus the host or the host cell appears to be of critical importance in determining the outcome of coronavirus infection. This report will focus on the role of the host cell in coronavirus replication and on the mutually dependent changes induced in the host cell and the virus during the establishment of a persistent infection in vitro.


Rough Endoplasmic Reticulum Persistent Infection Infectious Bronchitis Virus Wild Type Virus Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Behnke, J.N. and Holmes, K.V., 1978, Macromolecular Synthesis of a Temperature Sensitive Mutant of Mouse Hepatitis Virus., Abstr. Amer. Soc. Microbiol., p. 251.Google Scholar
  2. Doller, E.W. and Holmes, K.V., 1980, Different Intracellular Transportation of the Envelope Glycoproteins El and E2 of the Corona-virus MHV Abstr. Amer. Soc. Microbiol., p. 267.Google Scholar
  3. Evans, M.R. and Simpson, R.W., 1980, The Coronavirus Avian Infectious Bronchitis Virus Requires the Cell Nucleus and Host Trans-criptional Factors, Virol 105: 582.CrossRefGoogle Scholar
  4. Friedman, R.M. and Ramseur, J.M., 1979, Mechanisms of Persistent Infections by Cytopathic Viruses in Tissue Culture. Arch. Virol., 60: 83.PubMedCrossRefGoogle Scholar
  5. Holland, J.J., Grabau, E.A., Jones, C.L., and Semler, B.L., 1979, Evolution of Multiple Genome Mutations during Long Term Persistent Infection by Vesicular Stomatitis Virus, Cell 16: 495.PubMedCrossRefGoogle Scholar
  6. Holmes, K.V., 1978, Persistent Infection with a Coronavirus, IV Internat. Congr. Virol. Abstr., p. 453.Google Scholar
  7. Kang, C Y. and Allen, R., 1978, Host Function-Dependent Induction of Defective Interfering Particles of Vesicular Stomatitis Virus, J. Virol. 25: 202PubMedGoogle Scholar
  8. Lucas, A., Coulter, M., Anderson, R. and Dales, S., 1978, In Vivo and In Vitro Models of Demyelinating Diseases. II. Persistence and Host Regulated Thermosensitivity in Cells of Neural Derivation Infected with Mouse Hepatitis and Measles Viruses, Virol. 88: 325.CrossRefGoogle Scholar
  9. Sekellick, M.J. and Marcus, P.I., 1979, Persistent Infection II. Interferon-Inducing Temperature-Sensitive Mutants as Mediators of Cell Sparing: Possible Role in Persistent Infection by Vesicular Stomatitis Virus, Virol. 95: 36.CrossRefGoogle Scholar
  10. Sturman, L.S. and Takemoto, K.K., 1972, Enhanced Growth of a Murine Coronavirus in Transformed Mouse Cells, Infect. Immun. 6: 501.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Kathryn V. Holmes
    • 1
  • James N. Behnke
    • 1
  1. 1.Pathology Dept., Uniformed ServicesUniversity of the Health SciencesBethesdaUSA

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