Advertisement

Intracellular Protein Synthesis and the inVitro Translation of Coronavirus JHM mRNA

  • Stuart Siddell
  • Helmut Wege
  • Andrea Barthel
  • Volker ter Meulen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)

Abstract

The genome of the murine coronavirus JHM is a single-stranded, non-seamented RNA with a molecular weight of 5.4 — 6.7 × 10. The genomic RNA is infectious and at least one third is polyadenylated (Lai and Stohlman, 1978; Wege et al., 1978). The virion contains six proteins, four of which are glycosylated (Wege et al., 1979; Siddell et al., 1981b), including high and low molecular weight glycoproteins and a phosphorylated nucleocapsid protein of 60,000 molecular weight (Stohlman and Lai, 1979; Siddell et al., 1981a). Associated with purified virions is a protein kinase activity which specifically phosphorylates pp60 in vitro (Siddell et al., 1981a).

Keywords

Translation Product Rabbit Reticulocyte Lysate Mouse Hepatitis Virus Chase Period General Virology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson, R., Cheley, S. and Haworth-Hatherell, H., (1979). Comparison of polypeptides of two strains of murine hepatitis virus. Virology 97, 492–494.PubMedCrossRefGoogle Scholar
  2. Bond, C.W., Leibowitz, J.L., and Robb, J.A., (1979). Pathogenetic murine coronaviruses. II. Characterization of virus specific proteins of murine coronaviruses JHMV and A59V. Virology 94, 371–384.PubMedCrossRefGoogle Scholar
  3. Cavanagh, D. (1981). Structural polypeptides of coronavirus IBV. Journal of General Virology, in the press.Google Scholar
  4. Garwes, D.J. and Pocock, D.H. (1975). The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29, 25–34.PubMedCrossRefGoogle Scholar
  5. Laemmli, U.K., (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685.CrossRefGoogle Scholar
  6. Lai, M.M.C. and Stohlman, S.A. (1978). RNA of mouse hepatitis virus. Journal of Virology 26: 236–248.PubMedGoogle Scholar
  7. Macnaughton, M.R. (1980). The polypeptides of human and mouse coronaviruses. Archives of Virology 63, 75–80.PubMedCrossRefGoogle Scholar
  8. Pocock, D.J. and Garwes, D.H. (1977). The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. Journal of Virology 37, 487–499.Google Scholar
  9. Siddell, S.G., Wege H., Barthel, A. and ter Meulen, V. (1980). Coronavirus JHM: Cell-free Synthesis of Structural protein p 60. Journal of Virology 33, 10–17.PubMedGoogle Scholar
  10. Siddell, S.G., Barthel, A. and ter Meulen, V. (1981a). Coronavirus JHM. A virion-associated protein kinase Journal of General Virology, in the press.Google Scholar
  11. Siddell, S.G., Wege, H., Barthel, A. and ter Meulen, V. (1981b). Coronavirus JHM. Intracellular protein synthesis. Journal of General Virology, in the press.Google Scholar
  12. Smith, A.E., Wheeler, T., Glanville, N. and Kääriäinen, L. (1974). Translation of Semliki Forest virus 42S RNA in a mouse cell-free system to give virus coat proteins. European Journal of Biochemistry 49, 101–110.PubMedCrossRefGoogle Scholar
  13. Spaan, W.J.M., Rottier, P.J.M., Horzinek, M.C. and van der Zeijst, B.A.M. (1981). Isolation and identification of virus specific mRNA’s in cells infected with mouse hepatitis virus (MHV-A59). Virology, in the press.Google Scholar
  14. Stern, D.F. and Kennedy, S.I.T. (1980a). The coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA. Journal of Virology 34, 665–674.PubMedGoogle Scholar
  15. Stern, D.F. and Kennedy, S.I.T. (1980b). Coronavirus Multiplication Strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. Journal of Virology 36, 440–449.PubMedGoogle Scholar
  16. Stohlman, S.A. and Lai, M.M.C. (1979). Phosphoproteins of murine hepatitis viruses. Journal of Virology 32, 672–675.PubMedGoogle Scholar
  17. Sturman, L.S. (1977). Characterization of a coronavirus. I. Structural proteins: effect of preparative conditions on the migration of protein in polyacrylamide gels. Virology 77, 637–649.PubMedCrossRefGoogle Scholar
  18. Sturman, L.S. and Holmes, K.V. (1977). Characterization of a coronavirus. II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology 77, 650–660.PubMedCrossRefGoogle Scholar
  19. Wege, H., Müller, A. and ter Meulen, V. (1978). Genomic RNA of the murine coronavirus JHM. Journal of General Virology 41, 217–227.PubMedCrossRefGoogle Scholar
  20. Wege, H., Wege, H., Nagashima, K., and ter Meulen, V. (1979). Structural polypeptides of the murine coronavirus JHM. Journal of General Virology 42, 37–47.PubMedCrossRefGoogle Scholar
  21. Wege, H., Siddell, S.G., Sturm, M. and ter Meulen, V. (1981). Coronavirus JHM. Characterization of intracellular viral RNA, Journal of General Virology, in the press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Stuart Siddell
    • 1
  • Helmut Wege
    • 1
  • Andrea Barthel
    • 1
  • Volker ter Meulen
    • 1
  1. 1.Institute of VirologyUniversity of WürzburgGermany

Personalised recommendations