On Enteropathogenic Bovine Coronavirus
Cornonaviruses cause severe and economically important intestinal infections in newborn calves which become manifest as enteritis with diarrhea and additional clinical consequences. Studies concerning the pathogenesis of enteropathogenic bovine coronavirus (EBC-virus) enteritis have led to a better understanding of morphological alterations of the intestinal tract as well as of the individual cells, helping to explain the functional disorders observed with this disease (for references see Storz and Rott, 1980). Relatively little is known, however, about structural details of the virus and about the biochemical events of EBC-virus replication, principally a result of the difficulties encountered in growing the virus in tissue culture. After we succeeded in adapting EBC-virus strain L-9 isolated by Mebus et al. (1973) to grow in bovine fetal thyroid or brain cells in reasonable amounts it became possible to study some of the many unanswered questions. As a beginning, efforts were made to determine optimal growth conditions in order to provide an in vitro source for viral antigen. In addition, biochemical analysis of the virus was undertaken.
KeywordsInfected Cell Culture Feline Infectious Peritonitis Bovine Seron Feline Infectious Peritonitis Virus Serum Neutralization Test
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