Oligonucleotide Fingerprinting of the RNA of Different Strains of Infectious Bronchitis Virus

  • Jon P. Clewley
  • John Morser
  • Béla Lomniczi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


Infectious bronchitis virus /IBV/, the prototype of the family coronaviridae1 causes pathological conditions of the respiratory tract, the reproductive tract and the kidneys of chickens2. The disease was first described in the United States3, but by the early sixties it had been identified all over the world. Early work showed that more than one serotype of IBV existed4. Since then a large number of strains have been isolated and attempts have been made to classify these strains using a serological approach. On the basis of immunofluorescence data, and virus neutralization tests carried out mostly with reference strains, at least eight serotypes5 were established. However, in a study where large numbers of field strains were examined and compared to reference strains, it became apparent that it was not feasible to classify IBV isolates using a serological approach6. In an attempt to circumvent this problem strains were examined by cluster analysis using Euclidean distance as the measure of similarity and two main groups were established7. It was also found that twelve strains, fell into one or other of two categories of protein pattern differing in the mobility of a small glycoprotein on polyacrylamide gels8,9. It is of interest that reference strains placed in different groups by the cluster analysis also showed different protein patterns8.


Infectious Bronchitis Virus Live Vaccine Fingerprint Analysis Early Sixty Virus Neutralization Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D.A.J. Tyrell, D.J. Alexander, J.D. Almeida, C.H. Cunningham, B.C. Easterday, D.J. Garwes, J.C. Hierholzer, A. Kapikian, M.R. MacNaughton, and K. McIntosh, Coronaviridae: second report, Intervirol. 10: 321 /1978/.CrossRefGoogle Scholar
  2. 2.
    C.H. Cunningham, Avian infectious bronchitis, Adv. Vet. Sci. Comp. Med. 14: 105 /1970/.PubMedGoogle Scholar
  3. 3.
    A.P. Schalk, and M.C. Hawn, An apparently new respiratory disease of baby chicks, J. Amer. Vet. Med. Ass. 78: 413 /1931/.Google Scholar
  4. 4.
    M.S. Hofstad, Antigenic differences among isolates of avian infectious bronchitis virus, Amer. J. Vet. Res. 19: 740 /1958/.PubMedGoogle Scholar
  5. 5.
    S.R. Hopkins, Serological comparisons of strains of infectious bronchitis virus using plaque-purified isolants, Avian Dis. 18: 231 /1974/.PubMedCrossRefGoogle Scholar
  6. 6.
    P.S. Dawson, and R.E. Gough, Antigenic variation in strains of avian infectious bronchitis virus, Arch. g. Virusforsch. 34: 32 /1971/.CrossRefGoogle Scholar
  7. 7.
    J.G. Darbyshire, J.G. Rowell, J.K.A. Cook, and R.W. Peters, Taxonomic studies on strains of avian infectious bronchitis virus using neutralization tests in tracheal organ culture, Arch. Virol. 61: 227 /1979/.PubMedCrossRefGoogle Scholar
  8. 8.
    E. Nagy, and B. Lomniczi, Polypeptide patterns of infectious bronchitis virus serotype fall into two categories, Arch. Virol. 61: 341 /1979/.PubMedCrossRefGoogle Scholar
  9. 9.
    M.S. Collins, and D.J. Alexander, Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates. Arch. Virol. 63: 239 /1980/.PubMedCrossRefGoogle Scholar
  10. 10.
    J.P. Clewley, and D.H.L. Bishop, Oligonucleotide fingerprinting of viral genomes, in: “Newer T echniques in Practical Virology,” C.R. Harvard, ed., Alan R. Liss Inc., New York /in press/.Google Scholar
  11. 11.
    H. Van Roekel, K.L. Balls, M.K. Clarke, O.M. Olesiuk, and F.G. Sperling, Infectious bronchitis. Mass. Agric. Exp. Stn. Bull. No. 460. /1950/.Google Scholar
  12. 12.
    S.B. Hitchner, and P.G. White, Groth-curve studies of chick embryo-propagated IBV. Poultry Sci. 34: 590 /1955/.CrossRefGoogle Scholar
  13. 13.
    F.R. Beaudette, and C.B. Hudson, Cultivation of the virus of infectious bronchitis. J. Amer. Vet. Med. Ass. 90: 51 /1937/.Google Scholar
  14. 14.
    H.G. Purchase, C.H. Cunningham, and B.R. Burmaster, Identification and epizootiology of infectious bronchitis in a closed flock, Avian Dis. 10: 111 /1966/.CrossRefGoogle Scholar
  15. 15.
    J.W. Harkness, and C.D. Bracewell, Morphological variation among avian infectious bronchitis virus strains, Res. Vet. Sci. 16: 128 /1974/.PubMedGoogle Scholar
  16. 16.
    J. Hoekstra, and B. Rispens, Infectieuze bronchitis bij pluimvee. I. Laborat orium-experiment en met een sterkwerkende entstam, Tijdschr. Diergeneesk. 85: 398 /1960/.Google Scholar
  17. 17.
    G. Bijlenga, Het infectieuze bronchitis virus bij kiuken in Nederland, aangetoond mit behulp von ei-enting, dierexperimenteel onderzoek en serumneutralisatie-proveven, Tijdschr. Diergeneesk. 81: 43 /1956/.Google Scholar
  18. 18.
    S.R. Hopkins, Serological and immunological properties of a recent isolate of infectious bronchitis virus, Avian Dis. 13: 356 /1969/.PubMedCrossRefGoogle Scholar
  19. 19.
    E.L. Jungherr, T.W. Chomiak, and R.E. Luginbuhl, Immunologic differences in strains of infectious bronchitis virus, Proc. 60th Ann. Meeting U.S. Livestock San. Ass. Chicago. 203 /1956/.Google Scholar
  20. 20.
    B. Lomniczi, and L. Stipkovits, Isolation of the virus of infectious bronchitis, Magy. Áo. Lapja 23: 600 /1968/.Google Scholar
  21. 21.
    R.W. Winterfield and S.B. Hitchner, Etiology of an infectious nephritis-nephrosis syndrome of chickens, Amer. J. Vet. Res. 23: 1273 /1962/.PubMedGoogle Scholar
  22. 22.
    M.S. Hofstad, and H.W. Yoder, Jr., Avian infectious bronchitis — Virus distribution in tissues of chickens. Avian Dis. 10: 230 /1966/.PubMedCrossRefGoogle Scholar
  23. 23.
    R.B. Cummings, Infectious avian nephrosis /uraemia/ in Australia, Austral. Vet. J. 39: 145 /1963/.CrossRefGoogle Scholar
  24. 24.
    J.E. Lohr, Serological differences between strains of infectious bronchitis virus from New Zealand, Australia and the United States, Avian Dis. 20: 478 /1976/.CrossRefGoogle Scholar
  25. 25.
    B. Lomniczi, and I. Kennedy, Genome of infectious bronchitis virus. J. Virol. 24: 99 /1977/.PubMedGoogle Scholar
  26. 26.
    J.P. Clewley, J. Gentsch, and D.H.L. Bishop, Three unique species of snowshoe hare and LaCrosse bunyviruses. J. Virol. 22: 459 /1977/.PubMedGoogle Scholar
  27. 27.
    D.F. Stern, and S.I.T. Kennedy, Coronavirus multiplication strategy. I. Identification and characterization of virusspecified RNA. J. Virol. 34: 665 /1980/.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Jon P. Clewley
    • 1
  • John Morser
    • 1
  • Béla Lomniczi
    • 2
  1. 1.Department of Biological SciencesUniversity of WarwickCoventryUK
  2. 2.Veterinary Medical Research Institute of the Hungarian Academy of SciencesBudapestHungary

Personalised recommendations