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The Structure and Behavior of Coronavirus A59 Glycoproteins

  • Lawrence S. Sturman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)

Abstract

Coronavirus A59 contains three structural proteins. The location of these proteins in relation to the viral envelope and RNA are illustrated in the model shown in Figure 1. A nucleocapsid protein, N, (mw ≃ 50k) forms the nucleocapsid together with the RNA genome. Surrounding the nucleocapsid is a lipoprotein membrane which contains the envelope glycoproteins El (mw ≃ 23k) and E2 (mw ≃180k and 90k). El is an integral membrane protein, the bulk of which lies within the viral membrane and probably spans the lipid bilayer. E2 is a peripheral glycoprotein which forms the characteristic peplomers that are associated with this virion.

Keywords

Electrophoretic Mobility Viral Envelope Envelope Glycoprotein Nucleocapsid Protein Tris Maleate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alexander, D.J., and Collins, M.S., 1975, Effect of pH on the Growth and Cytopathogenicity of Avian Infectious Bronchitis Virus in Chick Kidney Cells, Arch. Virol., 49: 339.PubMedCrossRefGoogle Scholar
  2. Johnson, W.C., and Lindsey, A.J., 1939, An Improved Universal Buffer, Analyst, 64: 490.CrossRefGoogle Scholar
  3. Pocock, D.H., and Garwes, D.J., 1975, The Influence of pH on the Growth and Stability of Transmissible Gastroenteritis Virus in vitro, Arch. Virol., 49: 239.CrossRefGoogle Scholar
  4. Sturman, L.S., 1977, Characterization of a Coronavirus I. Structural Proteins: Effects of Preparative Conditions on the Migration of Protein in Polyacrylamide Gels, Virology, 77: 637.PubMedCrossRefGoogle Scholar
  5. Sturman, L.S., and Holmes, K.V., 1977, Characterization of a Coronavirus II. Glycoproteins of the Viral Envelope: Tryptic Peptide Analysis, Virology, 77: 650.PubMedCrossRefGoogle Scholar
  6. Sturman, L.S., Holmes, K.V., and Behnke, J., 1980, Isolation of Coronavirus Envelope Glycoproteins and Interaction with the Viral Nucleocapsid, J. Virol., 33: 449.PubMedGoogle Scholar
  7. Weitzman, S., Scott, V., and Keegstra, K., 1979, Analysis of Glycopeptides as Borate Complexes by Polyacrylamide Gel Electrophoresis, Anal. Biochem., 97: 438.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Lawrence S. Sturman
    • 1
  1. 1.Virus Laboratories Division of Laboratories and ResearchNew York State Department of HealthAlbanyUSA

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