Platelet Labelling in Atherosclerosis

  • Helmut Sinzinger
  • Irene Virgolini
  • Peter Fitscha
Part of the Nato ASI Series book series (NSSA, volume 219)


Three mechanisms are centrally involved in human atherogenesis, i.e., the role of the coagulation system: encrustation theory (C. von Rokitansky 1), cellular reaction to injury (Virchow 2), and lipid entry (Anitschkow 3) are well defined. Bizzozero (4) more than one century ago stressed the role of platelets. In recent years the early diagnosis of atherosclerosis using radioisotopic techniques has been frequently attempted. The most extensive information available concerns the involvement of the coagulation system using radiolabelling of either fibrinogen or platelets. In this brief overview all three of these processes of atherogenesis will be considered with special attention to use of labelled platelets. At present atherosclerosis can be functionally monitored at least partially to permit further insights into the development of the disease, but there are still limitations to be overcome.


Platelet Deposition Human Atherosclerotic Lesion Radioisotopic Technique Endothelial Coverage Platelet Labelling 
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  1. 1.
    C. von Rokitansky, “Über einige der wichtigsten Krankheiten der Arterien,” K.K. Hof-und Staatsdruckerei, Wien (1852).Google Scholar
  2. 2.
    R. Virchow, “Phlogose und Thrombose im Gefässystem. Gesammelte Abhandlungen zur wissenschaftlichen Medizin,” Hirsch, Berlin (1856).Google Scholar
  3. 3.
    N. N. Anitschkow, Experimental arteriosclerosis in animals, in: “Arteriosclerosis. A Survey of the Problem,” E. C. Cowdry, ed., MacMillan, New York (1933).Google Scholar
  4. 4.
    J. Bizzozero, Über einen neuen Formbestandteil des Blutes und dessen Rolle bei der Thrombose und der Blutgerinnung, Virchows Archiv Pathol. Anat. 90:261 (1882).CrossRefGoogle Scholar
  5. 5.
    H. Sinzinger and I. Virgolini, Nuclear medicine and atherosclerosis. A review, Eur. J. Nucl. Med. 31:in press (1990).Google Scholar
  6. 6.
    L. R. Erhardt, T. Lundman, and H. Mellstadt, Incorporation of 1251-labelled fibrinogen into coronary arterial thrombi in acute myocardial infarction in man. Lancet 1:387 (1973).PubMedCrossRefGoogle Scholar
  7. 7.
    A. Uehara, Y. Isaka, K. Hashikawa, K. Kimura, T. Kozuka, T. Kamada, H. Etani, S. Yoneda, and M. Imaizumi, 131-Iodine-labeled fibronectin: potential agent for imaging atherosclerotic lesion and thrombus, J. Nucl. Med. 29:1264 (1988).PubMedGoogle Scholar
  8. 8.
    M. L. Thakur, M. J. Welch, J. H. Joist, and R. E. Coleman, Indium-111 -labelled platelets: studies on preparation and evaluation of in vitro and in vivo functions, Thromb. Res. 9:345 (1976).PubMedCrossRefGoogle Scholar
  9. 9.
    H. Sinzinger, H. Kolbe, E. Strobl-Jäger, and R. Höfer, A simple and safe technique for sterile autologous platelet labelling using “Monovette” vials, Eur. J. Nucl. Med. 9:320 (1984).PubMedCrossRefGoogle Scholar
  10. 10.
    X. Wagner, S. Granegger, A. Dembinska-Kiec, and H. Sinzinger, Nitric oxide (NO) for radiolabelling of human platelets, in: “Radiolabelled Cellular Blood Elements,” H. Sinzinger and M. Thakur, eds., Facultas, Vienna (1989).Google Scholar
  11. 11.
    H. Sinzinger, K. Widhalm, J. Flores, and S. Granegger, Low density lipoproteins decrease labelling efficiency of human platelets, Nucl. Med. Commun. (submitted) (1990).Google Scholar
  12. 12.
    C. Kessler, R. Reuther, J. Berentelg, and B. Kimmig, The clinical use of platelet scintigraphy with 111-In-oxine, J. Neurol. 229:255 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    B. Pramsohler, G. Lupattelli, H. Scholz, and H. Sinzinger, Platelet scintigraphy and survival in juvenile stroke patients, in: “Radiolabelled Cellular Blood Elements,” H. Sinzinger and M. Thakur, eds., Alan R. Liss, Inc., New York, 71–80, (1990).Google Scholar
  14. 14.
    H. H. Davis, B. A. Siegel, L. A. Sherman, W. A. Heaton, T. P. Naidich, J. H. Joist, and M. J. Welch, Scintigraphic detection of carotid atherosclerosis with indium-111-labeled autologous platelets, J. Nucl. Med. 21:548 (1980).PubMedGoogle Scholar
  15. 15.
    R. S. Lees, A. M. Lees, and H. W. Strauss, External imaging of human atherosclerosis, J. Nucl. Med. 24:154 (1983).PubMedGoogle Scholar
  16. 16.
    J. Kaliman, H. Sinzinger, H. Bergmann, and C. Kolbe, Value of 123–1-low density lipoproteins (LDL) in the diagnosis of human atherosclerotic lesions, Circulation 72:300 (1985).Google Scholar
  17. 17.
    I. Virgolini, P. Angelberger, G. Lupattelli, J. Pidlich, and H. Sinzinger, In-vivo-Einstrom autologer radioaktiv markierter Low-density-Lipoproteine (LDL) in menschliche Gefässe, Wr. klin. Wschr. 101:798 (1989).Google Scholar
  18. 18.
    A. du P. Heyns, H. Pieters, and A. C. Steyn, Isolation and labeling with In-111 of a viable population of blood monocytes, in: “Radiolabelled Cellular Blood Elements,” H. Sinzinger and M. Thakur, eds., Alan R. Liss, Inc., New York, 261–270, (1990).Google Scholar
  19. 19.
    I. Virgolini, C. Müller, P. Fitscha, P. Chiba, and H. Sinzinger, Radiolabelling autologous monocytes with 111-Indium-oxine for reinjection in patients with atherosclerosis, in: “Radio- labelled Cellular Blood Elements,” H. Sinzinger and M. Thakur, eds., Alan R. Liss, Inc., New York, 271–280 (1990).Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Helmut Sinzinger
    • 1
    • 2
    • 3
  • Irene Virgolini
    • 1
    • 2
    • 3
  • Peter Fitscha
    • 1
    • 2
    • 3
  1. 1.Atherosclerosis Research Group (ASF)ViennaAustria
  2. 2.Atherosclerosis Research Group of the Austrian Academy of SciencesViennaAustria
  3. 3.2nd Department of Internal MedicinePoliclinic ViennaAustria

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