Glutathione Peroxidase, Selenium and Vitamin E in Defense Against Reactive Oxygen Species

  • Baldur Símonarson


In Mozart’s Magic Flute, the audience is misled as to the identity of heros and villains. At first, one is meant to believe that the Queen of the Night is a “goody”, whose daughter Pamina is held in the evil clutches of the “baddy” Sarastro. It turns out that Sarastro is a wise and kind man, while the Queen of the Night represents the powers of darkness and superstition. For much of this century, selenium was regarded only as a toxic element, and it comes as a surprise not only to the layman, but even to biochemists and physicians, that oxygen is potentially toxic. The reversal of the roles of selenium and oxygen is, however, not as dramatic as is that of the operatic characters.


Glutathione Peroxidase Selenium Level Glutathione Peroxidase Activity Maple Syrup Urine Disease Selenium Status 
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  1. Agergaard, N., and Jensen, P.T., 1982, Procedure for blood glutathione peroxidase determination in cattle and swine, Acta Vet. Stand., 23:515.Google Scholar
  2. Ammerman, C.B., and Miller, S.M., 1975, Selenium in ruminant nutrition: A review, J. Dairy Sci., 58:1561.PubMedCrossRefGoogle Scholar
  3. Anderson, P.H., and Patterson, D.S.P., 1976, Nutritional myopathy of young cattle, in “Proceedings, Roche Symposium for the Feed Industry,” Central Veterinary Laboratory, Weybridge.Google Scholar
  4. Anderson, P.H., Berrett, S., Catchpole, J., and Gregory, M.W., 1983, Effect of monensin on the selenium status of sheep, Vet. Rec., 113:498.PubMedCrossRefGoogle Scholar
  5. Beckett, G.J., Chapman, B.J., Dyson, E.H., and Hayes, J.D., 1985, Plasma glutathione S-transferase measurements after paracetamol overdose: evidence for early hepatocellular damage, Gut, 26:26.PubMedCrossRefGoogle Scholar
  6. Beilstein, M.A., and Whanger, P.D., 1983, Distribution of selenium and glutathione peroxidase in blood fractions from humans, rhesus and squirrel monkeys, rats and sheep, J. Nutr., 113:2138.PubMedGoogle Scholar
  7. Bergen, W.G., and Bates, D.B., 1984, Ionophres: Their effect on production efficiency and mode of action, J. Anim. Sci., 58:1465.PubMedGoogle Scholar
  8. Bieri, J.G., 1984, Vitamin E, in “Present Knowledge in Nutrition,” 5th edition, The Nutrition Foundation, Washington, D.C.Google Scholar
  9. Burk, R.F., 1983, Biological activity of selenium., Ann. Rev. Nutr., 3:53.CrossRefGoogle Scholar
  10. Burk, R.F., 1984, Selenium, in “Present Knowledge in Nutrition,” 5th edition, The Nutrition Foundation, Washington, D.C.Google Scholar
  11. Chen, X., Yang, G., Chen, J., Chen, X., Wen, Z., and Ge, K., 1980, Studies on the relations of selenium and Keshan disease, Biol. Trace Elem. Res., 2:91.CrossRefGoogle Scholar
  12. Combs, G.F., Jr., and Combs, S.B., 1984, The nutritional biochemistry of selenium, Ann. Rev. Nutr., 4:257.CrossRefGoogle Scholar
  13. Committee on Fetus and Newborn, 1985, Vitamin E and the prevention of retinopathy of prematurity, Pediatrics, 76:315.Google Scholar
  14. Cowgill, U.M., 1983, The distribution of selenium and cancer mortality in the continental United States, Biol. Tract Ehm. Res., 5:345.CrossRefGoogle Scholar
  15. Eiriksdóttir, G., Símonarson, B., Thorsteinsson, T., Gudmundsson, B., and Jónmundsson, J.V., 98, Árstídabundnar breytingar á seleni í blódi saudfjár. Tilraun á Hvanneyri 1980, Ísl. Landbún., 13:25 (with English summary).Google Scholar
  16. Epp, O., Ladenstein, R., and Wendel, A., 1983, The refined structure of the selenoenzyme glutathione peroxidase at 0.2-nm resolution, Eur. J. Biochem., 1133:51.CrossRefGoogle Scholar
  17. Fishbein, L., 1983, Environmental selenium and its significance, Fundam. Appl. Toxicol., 3:411.PubMedCrossRefGoogle Scholar
  18. Forward, R., and Almog, R., 1985, Separation of two forms of glutathione peroxidase from human erythrocytes by hydrophobic chromatography, J. Chromatogr., 330:383.PubMedCrossRefGoogle Scholar
  19. Glass, P., Avery, G.B., Subramanian, K.N.S., Keys, M.P., Sostek, A.M., and Friendly, D.S., 1985, Effect of bright light in the hospital nursery on the incidence of retinopathy of prematurity, New Engl. J. Med., 313:401. PubMedCrossRefGoogle Scholar
  20. Günzler, W.A., Kremers, H., and Flohé, L., 1974, An improved coupled test procedure for glutathione peroxidase (EC in blood, z. Klin. Chem. Klin. Biochem., 12:444.PubMedGoogle Scholar
  21. Günzler, W.A., Steffens, G.J., Grossmann, A., Kim, S.-M.A., Otting, F., Wendel, A., and Flohe, L., 1984, The amino acid sequence of bovine glutathione peroxidase, Hoppe-Seyler’s Z. Physiol. Chem., 365:195.PubMedCrossRefGoogle Scholar
  22. Halliwell, B., and Gutteridge, J.M.C., 1985, “Free Radicals in Biology and Medicine,” Clarendon Press, Oxford.Google Scholar
  23. Hoekstra, W.G., 1974, Biochemical role of selenium, in “Trace Element Metabolism in Animals — 2,” Hoekstra, W.G., Suttie, J.W., Ganther, H.E., and Mertz, W., eds., University Park Press, Baltimore.Google Scholar
  24. Hoekstra, W.G., 1975, Biochemical function of selenium and its relation to vitamin E., Fed. Proc., 34:2083.PubMedGoogle Scholar
  25. Howe, M., 1979, Selenium in the blood of South Dakotans, Arch. Environ. Health, 34:444.PubMedGoogle Scholar
  26. Kraus, R.J., Prohaska, J.R., and Ganther, H.E., 1980, Oxidized forms of ovine erythrocyte glutathione peroxidase. Cyanide inhibition of a 4-glutathione:4-selenoenzyme, Biochim. Biophys. Acta, 65:19.Google Scholar
  27. Låg, J., and Steinnes, E., 1978, Regional distribution of selenium and arsenic in humus layers of Norwegian forest soils, Feoderma, 20:3.Google Scholar
  28. Lawrence, R.A., and Burk, R.F., 1978, Species, tissue and subcellular distribution of non Sedependent glutathione peroxidase activity, J.Nutr., 108:211.Google Scholar
  29. Levander, O.A., and Morris, V.C., 1984, Dietary selenium levels needed to maintain balance in North American adults consuming self-selected diets, Am. J. Clin. Nutr., 39:809.PubMedGoogle Scholar
  30. Lo, M.-T, and Sandi, E., 1980, Selenium: Occurrence in foods and its toxicological significance — a review., J. Environ. Pathol. Toxicol., 4:193.PubMedGoogle Scholar
  31. Marshall, E., 1985, Selenium poisons refuge, Californian politics, Science, 229:144.PubMedCrossRefGoogle Scholar
  32. McKenzie, R.L., Rea, H.M., Thomson, C.D., and Robinson, M.F., 1978, Selenium concentration and glutathione peroxidase activity in blood of New Zealand infants and children, Am. J. Clin. Nutr., 31:1413.PubMedGoogle Scholar
  33. McMurray, C.H., and Blanchflower, W.J., 1979, Application of a high-performance liquid chromatographic fluorescence method for the rapid determination of α-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and high-performance liquid chromatography-ultraviolet detection methods, J. Chromatogr., 178:525.PubMedCrossRefGoogle Scholar
  34. McMurray, C.H., Rice, D.A., and Kennedy, S., 1983, Experimental models for nutritional myopathy, in “Biology of Vitamin E,” Ciba Foundation Symposium 101, Pitman, London.Google Scholar
  35. Mills, G.C., 1957, Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown, 2J. Biol. Chem., 229:189.Google Scholar
  36. Morris, J.S., Stampfer, M.J., and Willett, W., 1983, Dietary selenium in humans. Toenails as an indicator, Biol. Trace Elem. Res., 5:529.CrossRefGoogle Scholar
  37. Müller, A., Cadenas, E., Graf, P., and Sies, H., 1984, A novel biologically active seleno-organic compound — I. Glutathione peroxidase-like activity in vitro and anti-oxidant capacity of PZ 51 (Ebselen), Biochem. Pharmac, 33:3235.CrossRefGoogle Scholar
  38. Paglia, D.E., and Valentine, W.N., 1967, Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, J. Lab. Clin. Med., 70:158.PubMedGoogle Scholar
  39. Parnham, M.J., and Kindt, S., 1984, A novel biologically active seleno-organic compound — III. Effects of PZ 51 (Ebselen) on glutathione peroxidase and secretory activities of mouse macrophages, Biochem. Pharmac. 33:3247.CrossRefGoogle Scholar
  40. Piwonka, J., Kaiser, G., and Tölg, G., 1985, Determination of selenium at ng/g- and pg/g-levels by hydride generation atomic absorption spctrometry in biotic materials, Fresenius’ Z. Anal. Chem., 321:225.CrossRefGoogle Scholar
  41. Rea, H.M., Thomson, C.D., Campbell, D.R., and Robinson, M.F., 1979, Relationship between erythrocyte selenium concentrations and glutathione peroxidase (EC activities of New Zealand residents and visitors to New Zealand, Br. J. Nutr., 42:201.PubMedCrossRefGoogle Scholar
  42. Reddy, C.C., and Massaro, E.J., 1983, Biochemistry of selenium: A brief overview, Fundam. Appl. Toxicol., 3:431.PubMedCrossRefGoogle Scholar
  43. Riley, P.A., and Slater, T.F., 1969, Pathogenesis of retrolental fibropasia, Lancet, ii:265.CrossRefGoogle Scholar
  44. Robinson, M.F., Rea, H.M., Friend, G.M., Stewart, R.D.H., Snow, P.C., and Thomson, C.D., 1978, On supplementing the selenium intake of New Zealanders. 2. Prolonged metabolic experiments with daily supplements of selenomethionine, selenite and fish, Br. J. Nutr., 39:589.PubMedCrossRefGoogle Scholar
  45. Rotruck, J.T., Pope, A.L., Ganther, H.G., Swanson, A.B., Hafeman, D.G., and Hoekstra, W.G., 1973, Selenium: Biochemical role as a component of glutathione peroxidase, Science, 179:588.PubMedCrossRefGoogle Scholar
  46. Salonen, J.T., Alfthan, G., Huttunen, J.K., and Puska, P., 1984, Association between serum selenium and the risk of cancer, Am. J. Epidemiol. 120:342.PubMedGoogle Scholar
  47. Schwarz, K., and Foltz, C.M., 1957, Selenium as an integral part of factor 3 against dietary necrotic liver degeneration, J. Am. Chem. Soc., 79:3292.CrossRefGoogle Scholar
  48. Tappel, A.L., 1984, Selenium-glutathione peroxidase: Ptroperties and synthesis, in “Current Topics in Cellular Regulation,” Vol. 24, DeLuca, M., Lardy, H.A., and Cross, R.L., eds., Academic Press, New York.Google Scholar
  49. Thomson, C.D., and Robinson, M.F., 1980, Selenium in human health and disease with emphasis on those aspects peculiar to New Zealand, Am. J. Clin. Nutr., 33:303.PubMedGoogle Scholar
  50. Underwood, E.J., 1977, “Trace Elements in Human and Animal Nutrition,” 4th edition, Academic Press, New York.Google Scholar
  51. van Rij, A.M., Thomson, C.D., McKenzie, J.M., and Robinson, M.F., 1979, Selenium deficiency in total parenteral nutrition, Am. J. Clin. Nutr., 32:2076.PubMedGoogle Scholar
  52. van’t Hoff, J.H., 1967, “Imagination in Science”, Springer-Verlag, Berlin.CrossRefGoogle Scholar
  53. Watkinson, J.H., 1966, Fluorimetric determination of selenium in biological material with 2,3-diaminonaphthalene, Anal. Chem., 38:92.PubMedCrossRefGoogle Scholar
  54. Watkinson, J.H., 1981, Changes of blood selenium in New Zealand adults with time and importation of Australian wheat, Am. J. Clin. Nutr., 34:936.PubMedGoogle Scholar
  55. Wendel, A., 1980, Glutathione peroxidase, in “Enzymatic Basis of Detoxication,” Jakoby, W.B., ed., Academic Press, New York.Google Scholar
  56. Wendel, A., 1981, Glutathione peroxidase, Methods Enzymol., 77:325.PubMedCrossRefGoogle Scholar
  57. Wendel, A., Fausel, M., Safayhi, H., Tiegs, G., and Otter, R., 1984, A novel biologically active seleno-organic compound — II. Activity of PZ 51 in relation to glutathione peroxidase, Biochem. Pharmac., 33:3241.CrossRefGoogle Scholar
  58. Whanger, P.D., 1983, Selenium interactions with carcinogens, Fundam. Appl. Toxicol., 3:424.PubMedCrossRefGoogle Scholar

Suggestions for Further Reading

  1. Biology of Vitamin E, Ciba Foundation Symposium 101, 1983, Pitman, London.Google Scholar
  2. Chance, B., Sies, H., and Boveris, A., 1979, Hydroperoxide metabolism in mammalian organs, Physiol. Rev., 59:527.PubMedGoogle Scholar
  3. Flohe, L., 1979, Glutathione peroxidase: fact and fiction, in “Oxygen Free Radicals and Tissue Damage,” Ciba Foundation Symposium 65, Excerpta Medica, Amsterdam.Google Scholar
  4. Rosenfeld, I., and Beath, O.A., 1964, “Selenium. Geobotany, Biochemistry, Toxicity and Nutrition,” Academic Press, New York.Google Scholar
  5. Zingaro, R.A., and Cooper, W.C., eds., 1974, “Selenium,” Van Nostrand Reinhold Company, New York.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Baldur Símonarson
    • 1
  1. 1.Biochemistry Laboratory, Faculty of MedicineUniversity of Iceland, KeldurReykjavikIceland

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