Studies on the Renal Handling of Taurine: Changes during Maturation and After Altered Dietary Intake
The main route for the removal of taurine from the body is by urinary excretion. As a consequence, the renal tubular transport and handling of taurine is important in regulating the taurine content in the remainder of the body. In our previous report, we have described the use of stop-flow, free-flow micropuncture, continuous microperfusion and endogenous clearance as techniques to examine the renal handling of taurine (6). This chapter also covered our studies on the accumulation of taurine by thin cortex slices of mouse (4) and rat (5) kidney. These studies indicate that under normal conditions taurine excretion is 4 to 10% of the filtered load and that uptake by slices occurs by two processes: a low-Km, high-affinity uptake system and a high-Km, low-affinity uptake system. The kinetics of taurine accumulation are determined by Lineweaver-Burk analysis and the Eadie-Augustinson transformation. Other characteristics of taurine accumulation include transport by the β-amino acid transport system (such that β-amino acids but not α-amino acids will block uptake), sodium dependency of uptake, dependency on oxidative metabolism for active accumulation and enhanced efflux of taurine when incubated in a solution containing other β-amino acids.
KeywordsCreatinine Cysteine Luminal Methionine Taurine
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