Studies on the Renal Handling of Taurine: Changes during Maturation and After Altered Dietary Intake
The main route for the removal of taurine from the body is by urinary excretion. As a consequence, the renal tubular transport and handling of taurine is important in regulating the taurine content in the remainder of the body. In our previous report, we have described the use of stop-flow, free-flow micropuncture, continuous microperfusion and endogenous clearance as techniques to examine the renal handling of taurine (6). This chapter also covered our studies on the accumulation of taurine by thin cortex slices of mouse (4) and rat (5) kidney. These studies indicate that under normal conditions taurine excretion is 4 to 10% of the filtered load and that uptake by slices occurs by two processes: a low-Km, high-affinity uptake system and a high-Km, low-affinity uptake system. The kinetics of taurine accumulation are determined by Lineweaver-Burk analysis and the Eadie-Augustinson transformation. Other characteristics of taurine accumulation include transport by the β-amino acid transport system (such that β-amino acids but not α-amino acids will block uptake), sodium dependency of uptake, dependency on oxidative metabolism for active accumulation and enhanced efflux of taurine when incubated in a solution containing other β-amino acids.
KeywordsBrush Border Membrane Vesicle Renal Handling Taurine Transport Cortex Slice Taurine Concentration
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- 3.Burg, M.B., and Orloff, J., 1962, Oxygen consumption and active transport in separated renal tubules, Am. J. Physiol, 203: 327330.Google Scholar
- 6.Chesney, R.W., Jax, D.K., Scriver, C.R., and Mohyuddin, F., Taurine transport in mammalian kidney, in: “Taurine in Neurological Disorders,” R. Huxtable, and A. Barbeau, eds., Raven Press, New York (1978), pp. 73–94.Google Scholar
- 7.Chesney, R.W., Jax, D.K., Mohyuddin, F., and Scriver, C.R., 1978, In vitro use of polyethylene–(1,214C)-glycol to measure extra-cellular space in renal cortex slices from neonatal, immature and adult animals, Renal Physiol, 1: 166–170.Google Scholar
- 10.Chesney, R.W. and Jax, D.K., 1979, The influence of glutathione oxidation on renal cortex taurine transport, Life Sci, 25: 1497 1506.Google Scholar
- 11.Chesney, R.W., Friedman, A.L., Albright, P.W., and Gingery, R.R., The use of taurine as a model of developing amino acid transport processes, in: “Developmental Nephrology,” A. Spitzer, ed., in press.Google Scholar
- 13.Friedman, A.L., Jax, D.K., and Chesney, R.W., 1980, ß-amino acid transport in isolated tubule segments, Renal Physiol, 2: 21–28.Google Scholar
- 14.Friedman, A.L., Jax, D.K., and Chesney, R.W., Developmental aspects of renal ß-amino acid transport; III. Ontogeny of transport in isolated renal tubule segments, Ped. Research, in press.Google Scholar
- 15.Gingery, R., and Chesney, R.W., 1980, The influence of hypotaurine on taurine transport in isolated renal cortex tubules, Proc. of Soc. for Exp. Biol. and Med, 164: 18–22.Google Scholar
- 18.Lombardini, J.B., and Medina, E.V., 1978, Effects of dietary inorganic sulfate, taurine and methionine on tissue levels of taurine in the growing rat, J. of Nutrition, 108: 428–433.Google Scholar
- 19.Rogers, Q.R., and Harper, A.E., 1965, Amino acid diets and maximal growth in the rat, J. of Nutrition, 87: 267–273.Google Scholar
- 21.Steele, T.H., Underwood, J.L., Stronberg, B.A., and Larmore, C.A., 1976, Renal resistance to parathyroid hormone during phosphorus deprivation, J. Clin. Invest 58: 1461–14 64.Google Scholar
- 22.Sturman, J.A., 1973, Taurine pool sizes in the rat: Effects of vitamin B-6 deficiency and high-taurine diet, J. of Nutrition, 103: 1566–1580.Google Scholar