Taurine Transport by Reconstituted Membrane Vesicles

  • S. W. Schaffer
  • E. C. Kulakowski
  • J. H. Kramer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 139)


Although taurine is found in very high concentration in the heart, a role for this amino acid has not been established. Read and Welty (24) initially reported in 1963 that taurine exhibited antiarrhythmic activity; they attributed this effect of taurine to changes in potassium flux. Since that study taurine has also been linked to changes in calcium transport (26). In addition, several investigators have described specific membrane-stabilizing effects of taurine (10,16).


Taurine Transport Taurine Content Taurine Uptake Soybean Phospholipid Cysteine Sulfinic Acid 
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  1. 1.
    Awapara, J., and Berg, M., Uptake of taurine by slices of rat heart and kidney, in: “Taurine,” R. Huxtable and A. Barbeau, eds., Raven Press, New York (1976), pp. 135–143.Google Scholar
  2. 2.
    Azari, J., Brumbaugh, P., and Huxtable, R., 1980, Prophylaxis by taurine in the hearts of cardiomyopathic hamsters, J. Mol. Cell. Cardiol., 12: 1353–1366.PubMedCrossRefGoogle Scholar
  3. 3.
    Bahl, J., Frangakis, C.J., Larsen, B., Chang, S., Grosso, D., and Bressler, R., Accumulation of taurine by isolated rat heart cells and rat heart slices, in: “Action of Taurine on Excitable Tissues,” S.W. Schaffer, J.J. Kocsis and S.I. Baskin, eds., Spectrum Press, New York, in press.Google Scholar
  4. 4.
    Chesney, R.W., and Jax, D.K., 1977, Heterogeneity of the 13-amino preferring transport system in rat kidney cortex, Biochim. Biophys. Acta., 466: 84–96.Google Scholar
  5. 5.
    Christensen, H.N., 1979, Exploiting amino acid structure to learn about membrane transport, Adv. in Enzymology, 49: 41–101.Google Scholar
  6. 6.
    Crass, III, M.F., and Lombardini, J.B., 1977, Loss of cardiac muscle taurine after left ventricular ischemia, Life Sciences, 21: 951–958.Google Scholar
  7. 7.
    Cuatrecasas, P., 1972, Isolation of the insulin receptor of liver and fat-cell membranes, Proc. Nat. Acad. Sci., U.S.A., 69: 318–322.CrossRefGoogle Scholar
  8. 8.
    Grosso, D.S., Roeske, W.R., and Bressler, R., 1978, Characterization of a carrier-mediated transport system for taurine in the fetal mouse heart in vitro, J. Clin. Invest., 61: 944–952.PubMedCrossRefGoogle Scholar
  9. 9.
    Hruska, R.E., Huxtable, R.J., and Yamamura, H.I., High-affinity, temperature-sensitive, and sodium-dependent transport of taurine in rat brain, in: “Taurine and Neurological Disorders,” A. Barbeau and R.J. Huxtable, eds., Raven Press, New York, (1978), pp. 109–117.Google Scholar
  10. 10.
    Huxtable, R., and Bressler, R., 1973, Effect of taurine on a muscle intracellular membrane, Biochim. Biophys. Acta, 323: 573–583.PubMedCrossRefGoogle Scholar
  11. 11.
    Huxtable, R., and Bressler, R., 1974, Elevation of taurine in human congestive heart failure, Life Sciences, 14: 1353–1359.PubMedCrossRefGoogle Scholar
  12. 12.
    Huxtable, R.J., Chubb, J., and Azari, J., 1980,Physiological and experimental regulation of taurine content in the heart, Federation Proc., 39:2685–2690.Google Scholar
  13. 13.
    Huxtable, R.J., Laird, II, H.E., and Lippincott, S.E., 1979, The transport of taurine in the heart and the rapid depletion of tissue taurine content by guanidinoethyl sulfonate, J. Pharmacol. Exp. Ther., 211: 465–471.PubMedGoogle Scholar
  14. 14.
    Kagawa, Y., and Racker, E., 1971, Partial resolution of the enzyme catalyzing oxidative phosphorylation. XXV Reconstitution of vesicles catalyzing 32Pí - Adenosine Triphosphate exchange, J. Biol. Chem., 246: 5477–5487.Google Scholar
  15. 15.
    Kasahara, M., and Hinkle, P.C., 1977, Reconstitution and purification of the D-glucose transporter from human erythrocytes, J. Biol. Chem., 252: 7384–7390.PubMedGoogle Scholar
  16. 16.
    Kramer, J.H., Chovan, J.P., and Schaffer, S.W., 1981, The effect of taurine on calcium paradox and ischemic heart failure, Am. J. Physiol., in press.Google Scholar
  17. 17.
    Kulakowski, E., Maturo, J., and Schaffer, S.W., 1978, Identification of taurine binding receptors from rat heart sarcolemma, Biochem. Biophys. Res. Commun., 80: 936–941.PubMedCrossRefGoogle Scholar
  18. 18.
    Mitchell, P., 1979, Compartmentation and communication in living systems, ligand conduction: a general catalytic principle in chemical, osmotic and chemiosmotic reaction systems, Eur. J. Biochem., 95: 1–20.PubMedCrossRefGoogle Scholar
  19. 19.
    Nauss-Karol, Cheryl and Vanderwende, C., 1981, High affinity taurine uptake in human blood platelets, in: “Actions of Taurine on Excitable Tissues,” S.W. Schaffer, J.J. Kocsis and S.I. Baskin, eds., Spectrum Press, New York, in press.Google Scholar
  20. 20.
    Newman, W.H., Frangakis, C.J., Grosso, D.S. and Bressler, R., 1977, A relation between myocardial taurine content and pulmonary wedge pressure in dogs with heart failure, Physiol. Chem. and Physics, 9: 259–263.Google Scholar
  21. 21.
    Pasantes-Morales, H., Salceda, R., and López Colomê, A.M., Taurine in normal retina, in: “Taurine and Neurological Disorders”, A. Barbeau and R.J. Huxtable, eds., Raven Press, New York, (1978), pp. 265–279.Google Scholar
  22. 22.
    Peterson, M.B., Mead, R.J., and Welty, J.D., 1973, Free amino acids in congestive heart failure, J. Mol. Cell. Cardiol., 5: 139–147.CrossRefGoogle Scholar
  23. 23.
    Pressman, B.C., 1976, Biological applications of ionophores, Ann. Rev. Biochem., 45: 501–530.CrossRefGoogle Scholar
  24. 24.
    Read, W.O., and Welty, J.D., 1963, Effect of taurine on epinephrine and digoxin induced irregularities of the dog heart, J. Pharmacol. Exp. Ther., 139: 283–289.PubMedGoogle Scholar
  25. 25.
    Rozen, R., Tenenhouse, H.S., and Scriver, C.R., 1979, Taurine transport in renal brush-border membrane vesicles, Biochem. J., 180: 245–248.PubMedGoogle Scholar
  26. 26.
    Schaffer, S.W., Kramer, J., and Chovan, J.P., 1980, Regulation of calcium homeostasis in the heart by taurine, Federation Proc., 39: 2691–2694.Google Scholar
  27. 27.
    Schaffer, S.W., Safer, B., Scarpa, A., and Williamson, J.R., 1974, Mode of action of the calcium ionophores X-537A and A-23187 on cardiac contractility, Biochem. Pharmacol. 23: 1609–1617.PubMedCrossRefGoogle Scholar
  28. 28.
    Sulakhe, P.V., Drummond, G.I., and Ng, D.C., 1973, Calcium binding by skeletal muscle sarcolemma,J. Biol. Chem., 248: 4150–4157.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • S. W. Schaffer
    • 1
  • E. C. Kulakowski
    • 1
  • J. H. Kramer
    • 1
  1. 1.Department of PharmacologyUniversity of South AlabamaMobileUSA

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