Appendix to Calcium Exchange in the Heart
45Ca exchange was monitored by the scintillation-disk flow cell technique developed in the laboratories at UCLA, It depends upon the growth of a cellular monolayer on one surface of each of two disks composed of polystyrene combined with scintillator material (Bicron Corp., Newbury, Ohia, USA). The disks are 45 mm in diameter and 1 mm thick and are designed to form a portion of each side of the flow cell chamber illustrated in Figure 1.
KeywordsFlow Cell Perfusion Solution Single Port Mitochondrial Calcium Calcium Exchange
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