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Isotachophoretic Analysis of Some Compounds Involved in Energy Metabolism in Normal and Pathological Human Muscle Extracts

  • M. F. J. Brolsma
  • F. T. J. Oerlemans
  • M. P. Verburg
  • C. H. M. M. De Bruyn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

A number of muscular diseases seem to be associated with disturbances in purine metabolism. Altered purine metabolite compositions have been described for instance in Duchenne-type muscular dystrophy (DMD) (1) and malignant hyperthermia (2). For clarification of the possible metabolic defects as well as for diagnostic reasons it is important to know levels and variations in metabolites in muscle biopsies of control persons as well as patients suffering from muscular diseases. Isotachophoresis is a convenient technique for these analyses because, apart from nucleotides, other muscle metabolites important in energy metabolism such as orthophosphate (Pi), phosphocreatine (PCr) and lactate can be detected in a single run of about twenty minutes.

Keywords

Control Person Malignant Hyperthermia Energy Charge Caproic Acid Purine Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    F. Oerlemans, C. van Bennekom, C. de Bruyn and S. Kulakowski, Metabolite Profiling of Human Muscle Extracts by Isotachophoresis, J.Inher.Metab.Dis. 4: 104 (1981)CrossRefGoogle Scholar
  2. 2.
    C. A. v. Bennekom, F. Oerlemans, M. P. Verbürg and C. de Bruyn, Anal. Chem. Symp. series, Elsevier Scientific Comp. vol 7 in press.Google Scholar
  3. 3.
    D. C. Gower and R. C. Woledge, The Use of Isotachophoresis for Analysis of Muscle Extracts, Science Tools 24: 17 (1977)Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • M. F. J. Brolsma
    • 1
  • F. T. J. Oerlemans
    • 2
  • M. P. Verburg
    • 3
  • C. H. M. M. De Bruyn
    • 2
  1. 1.Guy’s HospitalLondonEngland
  2. 2.University Hospital St.RadboudNijmegenThe Netherlands
  3. 3.De Wever HospitalHeerlenThe Netherlands

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