Cell-Cycle Dependent Variation in the Levels of Deoxyribonucleoside Triphosphate in Mouse T-Lymphoma Cells
De novo synthesis of deoxyribonucleotides occurs exclusively by the reduction of the corresponding ribonucleotides in a reaction catalyzed by the enzyme ribonucleotide reductase. The enzyme is subject to allosteric regulation in vitro and in vivo and its activity is closely correlated to the growth rate of cells. The changes in ribonucleotide reduction is correlated to changes in the measured deoxyribonucleoside triphosphate pools in a variety of cell lines but considerable variations in the relative levels of each of the four DNA precursors were found1.
KeywordsRibonucleotide Reductase Thymidine Kinase Activity High Performance Liquid Chromato Deoxyribonucleoside Triphosphate Ribonucleoside Triphosphate
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- 2.S. Eriksson and D.W. Martin, Jr., Ribonucleotide reductase in cultured mouse lymphoma cells: cell cycle-dependent variation in the activity of subunit proteins M2, J.Biol.Chem. 256: 94–36 (1981).Google Scholar