Thymidine Sensitivity and Deoxynucleotide Pools of Human Lymphoid and Melanoma Cells in Vitro

  • A. Leyva
  • H. Appel
  • H. M. Pinedo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


Exposure of cells to excessive amounts of thymidine (dThd) may cause inhibition of DNA synthesis due to a depletion of deoxycytidine 5′-triphosphate (dCTP). Thymidine 5′-triphosphate (dTTP) accumulates in dThd-treated cells and leads to inhibition of ribonucleotide reductase-mediated synthesis of cytosine deoxynucleotides1. The toxic effects of dThd have been investigated with various mammalian cells in vitro. Notably, lymphoid cells of T-cell origin, but not of B-cell origin, are highly sensitive to dThd2,3. In vitro and in vivo studies have been reported demonstrating the therapeutic potential of high-dose dThd treatment against melanoma4–6. However, no deoxynucleotide metabolism studies on melanoma cells have been reported. In the present study we compared human T- and B- cells and melanoma cells in vitro with respect to dThd sensitivity, deoxynucleotide pool profiles and changes in deoxynucleotide levels in response to dThd.


Melanoma Cell High Pressure Liquid Chromatography Nucleotide Pool NC37 Cell Pool Profile 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • A. Leyva
    • 1
    • 2
  • H. Appel
    • 1
    • 2
  • H. M. Pinedo
    • 1
    • 2
  1. 1.Section of Experimental Chemotherapy, Netherlands Cancer InstituteFree University HospitalAmsterdamThe Netherlands
  2. 2.Department of OncologyFree University HospitalAmsterdamThe Netherlands

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