Deoxyadenosine Induced G1 Phase Arrest in Leukemic T Cells

  • Richard M. Fox
  • Edith H. Tripp
  • Ian W. Taylor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


The recognition that human combined immune deficiency is associated with inborn adenosine deaminase (ADA) deficiency has focused attention on the importance of purine nucleoside metabolism and toxicity in the lymphoid system1. This has been reinforced by the development of drugs which inhibit ADA, in particular deoxy-coformycin (DCF), now used in the treatment of T cell and other leukemias2. Cultured human T leukemic lymphoblasts (in the presence of ADA inhibitors) are highly sensitive to dAdo induced cytotoxicity; while Epstein-Barr Virus (EBV) transformed B cell lines are resistant. These cell lines have proven valuable models to study dAdo metabolism and toxicity3.


Deoxycytidine Kinase Centrifugal Elutriation Adenine Arabinoside Human Acute Lymphoblastic Leukemia Flow Cytometric Cell Cycle Analysis 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Richard M. Fox
    • 1
  • Edith H. Tripp
    • 1
  • Ian W. Taylor
    • 1
  1. 1.Ludwig Institute for Cancer Research (Sydney Branch) Blackburn BuildingUniversity of Sydney. N.S.W. 2006Australia

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