Abstract
The recognition that human combined immune deficiency is associated with inborn adenosine deaminase (ADA) deficiency has focused attention on the importance of purine nucleoside metabolism and toxicity in the lymphoid system1. This has been reinforced by the development of drugs which inhibit ADA, in particular deoxy-coformycin (DCF), now used in the treatment of T cell and other leukemias2. Cultured human T leukemic lymphoblasts (in the presence of ADA inhibitors) are highly sensitive to dAdo induced cytotoxicity; while Epstein-Barr Virus (EBV) transformed B cell lines are resistant. These cell lines have proven valuable models to study dAdo metabolism and toxicity3.
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References
J. E. Seegmiller, L. Thompson, R. Willis, S. Matsumoto, D. Carson. Nucleotide and nucleoside metabolism and lymphocyte function. In Biological Basis of Immunodeficiency. E. W. Gelfand, H. M. Dosch (eds) Raven Press. New York. p. 251 (1980)
H. G. Prentice, K. Ganeshagaru, K. F. Bradstock, A. H. Goldstone, J. F. Smyth, B. Wonke, G. Janossy, A. V. Hoffbrand. Remission induction with adenosine deaminse inhibitor 2′deoxycoformycin in Thy-lymphoblastic leukemia. Lancet. 2: 170 (1980).
R. M. Fox, S. K. Piddington, E. H. Tripp, M. H. N. Tattersall. Ecto-adenosine triphosphate deficiency in cultured human T and Null leukemic lymphocytes. A biochemical basis for thymidine sensitivity. J. Clin. Invest. 68: 544 (1981).
M. S. Hershfield. Apparent suicide inactivation of human lymphoblast S-adenosylhomocysteine hydrolase by 2′deoxy-adenosine and adenine arabinoside. J. Biol. Chem. 254: 22 (1979).
R. M. Bohmer. Flow cytometric cell cycle analysis using the quenching of 33258 Hoechst fluorescence by bromouridine incorporation. Cell Tissue Kinet. 12: 101 (1979).
B. K. Milthorpe. FMFPAKI: A program package for routine analysis of single parameter flow microfluorimetric data on a low cost mini-computer. Comput. Biomed. Res. 13: 417 (1980).
A. A. Piper, M. H. N. Tattersall, R. M. Fox. The activities of thymidine metabolising enzymes during the cell cycle of a human lymphocyte cell line LAZ-007 synchronised by centrifugal elutriation. Biochim. Biophy. Acta. 633: 400 (1980).
T. A. Krenitsky, J. V. Tuttle, G. W. Koszalka, I. S. Chen, L. M. Beacham III, J. L. Ridout, G. B. Elion. Deoxycytidine kinase from calf thymus. Substrate and inhibitor specificity. J. Biol. Chem. 251: 4055 (1976).
M. S. Hershfield, J. E. Fetter, W. C. Small, A. S. Bagnara, S. R. Williams, B. Ullman, D. W. Martin Jnr., D. B. Watson and D. A. Carson. Effects of mutational loss of adenosine kinase and deoxycytidine kinase on deoxyATP accumulation and deoxy-adenosine toxicity in cultured CEM human T-lymphoblastoid cells. J. Biol. Chem (in press).
A. B. Pardee, R. Dubrow, J. L. Hamlin, R. F. Kletzein. Animal cell cycle. Ann. Rev. Biochem. 47: 715 (1978).
P. Coffino, J. W. Graw, W. Tomkins. Cyclic-AMP, a nonessential regulator of the cell cycle. Proc. Natl. Acad. Sci. U.S.A. 72: 878 (1975).
E. B. Thompson, J. B. Harmon, M. R. Norman, T. J. Schmidt. Glucocorticoid actions in a human acute lymphoblastic leukemia T cell line: A model system for understanding toxicity. In Hormones and Cancer. S. Iacobelli, R. J. B. King, H. R. Lidner, M. E. Lippman (eds). Raven Press. New York. p. 89 (1980).
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Fox, R.M., Tripp, E.H., Taylor, I.W. (1984). Deoxyadenosine Induced G1 Phase Arrest in Leukemic T Cells. In: De Bruyn, C.H.M.M., Simmonds, H.A., Müller, M.M. (eds) Purine Metabolism in Man-IV. Advances in Experimental Medicine and Biology, vol 165. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0390-0_63
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DOI: https://doi.org/10.1007/978-1-4757-0390-0_63
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