Sensitivity of T-Leukemic Cells to Deoxyguanosine and Arabinosyl Guanine
The association of profound immunodeficiency with deficiencies of the purine enzymes adenosine deaminase (ADA) and purine nucleoside Phosphorylase (PNP) has revealed the important role of these metabolic pathways in the differentiation, maturation and proliferation of lymphoid cells.1 The selective interference of certain T-lymphocyte functions in PNP deficiency2 and the elevation of deoxyguanosine triphosphate (deoxyGTP) in patient erythrocytes3 has focused attention on the potential role of deoxyguanosine (GdR) in the pathogenesis of these events. Our initial studies identified significant differences between immature and mature T-cells in their response to GdR. Following incubation with GdR, immature T-cells fail to proliferate, rapidly accumulate deoxyGTP, and fail to eliminate the nucleotide.4
KeywordsRibonucleotide Reductase Purine Nucleoside Phosphorylase Chronic Myelogenous Leukemic Cell Deoxycytidine Kinase Adenosine Deaminase Activity
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