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Biochemical Basis for the Differential Effects of Deoxycoformycin on Human Leukemias

  • A. L. Yu
  • S. Matsumoto
  • L. Bleeker
  • A. Alvarez
  • B. Bakay
  • W. L. Nyhan
  • F. Kung
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

T-cell and null cell lines cultured in vitro are more sensitive to 2′-deoxycoformycin (DCF), an inhibitor of adenosine deaminase (ADA), plus 2′-deoxyadenosine (dAdo) than are B-cell lines1,2. The malignant, monoclonal cells of leukemia patients span a wide range of immunological types. This study compares the sensitivity of the various immunologic subtypes of leukemic cells to DCF plus dAdo and investigates the biochemical basis of the differential sensitivity. The information obtained may be of value in identifying the subtypes of leukemia that respond clinically to this drug.

Keywords

Leukemic Cell Thymidine Incorporation Adenosine Deaminase Biochemical Basis Null Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    R.M. Fox, S.K. Piddington, E.H. Tripp, M.H.N. Tattersall, Ecto-adenosine triphosphatase deficiency in cultured human T and null leukemic lymphocytes. J. Clin. Invest. 68:544, 1981.Google Scholar
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    D.A. Carson, J. Kaye, D.B. Wasson, The potential importance of soluble deoxynucleotidase activity in mediating deoxyadenosine toxicity in human lymphoblasts. J. Immunol. 126:348, 1981.PubMedGoogle Scholar
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    M.F.E. Siaw, B.S. Mitchell, CA. Koller, M.S. Coleman, J.J. Hutton, ATP depletion as a consequence of adenosine deaminase inhibition in man. Proc. Natl. Acad. Sci. USA 77:6157, 1980.PubMedCrossRefGoogle Scholar
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    A.L. Yu, B. Bakay, F.H. Kung, W.L. Nyhan, Effects of 2′-deoxycoformycin on the metabolism of purines and the survival of malignant cells in a patient with T-cell leukemia. Cancer Res 41:2677, 1981.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • A. L. Yu
    • 1
  • S. Matsumoto
    • 1
  • L. Bleeker
    • 1
  • A. Alvarez
    • 1
  • B. Bakay
    • 1
  • W. L. Nyhan
    • 1
  • F. Kung
    • 1
  1. 1.Department of PediatricsUniversity of CaliforniaSan Diego, La JollaUSA

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