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Uptake of Thymidine by Plasma Membrane Vesicles of L5178Y Murine Leukemic Cells

  • M. Torrent-Quetglas
  • M. R. Grau-Oliete
  • M. P. Rivera-Fillat
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

Purified plasma membrane vesicles possess considerable advantages over whole cells for the study of the transport of nutrients regardless of intracellular metabolism or binding of the transported substances to intracellular structures.

Keywords

Equilibrium Exchange Thymidine Uptake Plasma Membrane Vesicle Thin Layer Chromatogra Murine Leukemic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    M. Torrent-Quetglas, M. P. Rivera-Fillat, and M. R. Grau-Oliete, Murine Leukemic Lymphoblasts: Homogenization and Fractionation Procedures for Plasma Membrane Vesicles Isolation, Anal. Biochem. 114: 228 (1981).PubMedCrossRefGoogle Scholar
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    G. R. Schacterle, and P. L. Pollack, A Simplified Method for the Quantitative Assay of Small Amounts of Protein in Biological Material, Anal. Biochem. 51: 654 (1973).PubMedCrossRefGoogle Scholar
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    V. E. Honegger, S. S. Bogdanov, and P. R. Bally, Quantitative Extraction, Separation and Recovery of Adenine-Derived Radioactivity in Bases, Nucleosides and Nucleotides from Blood Platelets using PEI-Cellulose Thin-Layer Chromatography, Anal. Biochem. 81: 268 (1977).PubMedCrossRefGoogle Scholar
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    Y. Eylam, and W. D. Stein, Kinetic Studies of Transport across Red Blood Cell Membranes, in: “Methods in Membrane Biology”, E. D. Korn, ed., Plenum Press, New York (1974).Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • M. Torrent-Quetglas
    • 1
  • M. R. Grau-Oliete
    • 1
  • M. P. Rivera-Fillat
    • 1
  1. 1.Instituto de FarmacologíaC.S.I.C.BarcelonaEspaña

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