Advertisement

5′Nucleotidase and Adenosine Deaminase Activities in Human Lymphocytes and Lymphoblastoid Cell Lines

  • Jacques Dornand
  • Jean-Claude Bonnafous
  • Jean Favero
  • Agnés Gartner
  • Jean-Claude Mani
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

The adenosine generating enzyme 5′nucleotidase (5′N) is located on the external surface of most mammalian cells including lymphocytes1. Recent studies indicate that different lymphoid cells have widely different 5′N levels; some discrepancies appear among these data, which certainly result from different methods of 5′N determination2, but low 5′N activities were unambiguously found in lymphocytes from patients with CLL3 or hypogammaglobulinemia4 and in cord blood lymphocytes5. It was postulated that 5′N deficiency could reflect an early stage of maturation, hypothesis which is emphasized by the fact that low 5′N activities were reported for thymocytes from various species6–9. We previously demonstrated that in mouse this low level of 5′N is due to the high percentage of peanut agglutinin (PNA)-positive, hydrocortisone-sensitive thymocyte population, which represents immature cortical cells; on the contrary PNA-negative thymocytes, which are immunocompetent and represent the medullary mature population, display a 10-fold higher 5′N level than PNA+ thymocytes10. This report was conflicting with that of Barton et at 11 who found no difference in 5′N activities between rat medullary and cortical thymocytes. A recent report of Cohen et al 12, showing that 5′N activity of unseparated human thymocytes increased after incubation with factors known to induce thymocyte maturation, prompted us to verify if immature human thymocytes also lack 5′N activity like immature mouse thymocytes.

Keywords

Peripheral Blood Lymphocyte Adenosine Deaminase Burkitt Lymphoma Lymphoblastoid Cell Line Human Thymocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    J. Dornand, C. Reminiac and J.C. Mani, Biochimie 59: 425 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Dornand, J.C. Bonnafous, J. Favero and J.C. Mani, Biochem. Med. (1982 in press).Google Scholar
  3. 3.
    F. Quagliata, D. Faig, M. Conklyn and R. Silber, Cancer Res. 34: 3197 (1974).PubMedGoogle Scholar
  4. 4.
    S.M. Johnson, M.E. North, G.L. Asherson, J. Allsop, R.W. Watts and A.D.B. Webster, Lancet i: 168 (1977).CrossRefGoogle Scholar
  5. 5.
    M. Rowe, G.C. De Gast, T.A.E. Platts-Mills, G.L. Asherson, A.D.B. Webster and S.M. Johnson, Clin. Exp. Immunol. 39: 337 (1980).PubMedGoogle Scholar
  6. 6.
    D.N. Misra, C.T. Ladoulis, L.W. Estes and T.J. Gill III, Biochemistry 14: 3014 (1975).PubMedCrossRefGoogle Scholar
  7. 7.
    R.J. Uusitalo and M.J. Karnovsky, J. Histochem. Cytochem. 25: 87 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    S.H. Gregory and M. Kern, J. Immunol. 123: 1078 (1979).PubMedGoogle Scholar
  9. 9.
    A. Cohen, W.W.J. Lee, H.M. Dosch and W.E. Gelfand, J. Immunol. 125: 1578 (1981).Google Scholar
  10. 10.
    J. Dornand, J.C. Bonnafous and J.C. Mani, FEBS Lett. 118: 225 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    R.W. Barton and I. Goldschneider, J. Immunol. 121: 2329 (1979).Google Scholar
  12. 12.
    A. Cohen, H.M. Dosch and E.W. Gelfand, Clin. Immunol. Immuno-pathol. 18: 287 (1981).CrossRefGoogle Scholar
  13. 13.
    J. Dornand, J.C. Bonnafous, J. Favero and J.C. Mani, FEBS Lett. (1982 in press)Google Scholar
  14. 14.
    Y. Reisner, M. Biniaminov, E. Rosenthal, N. Sharon and B. Ramot, Proc. Natl. Acad. Sci. USA 76: 447 (1979).PubMedCrossRefGoogle Scholar
  15. 15.
    J.C. Bonnafous, J. Dornand and J.C. Mani, Biochim. Biophys. Acta (1982 in press)Google Scholar
  16. 16.
    J. Dornand, J.C. Bonnafous, J. Favero, I. Dauthuille, A. Gartner, P. Poncelet, P. Carayon, B. Pau and J.C. Mani, in: “Fluorescent Technique and Membrane Markers in Cancer and Immunology: Membrane Dynamics, Cellular Characterization and Cell Sorting”, C. Rosenfeld, B. Serrou and P. Viallet eds., (1982 in press).Google Scholar
  17. 17.
    H. Lazarus, E.F. Barel, S. Oppenheim and A. Krishan, In Vitro 9: 303 (1974).CrossRefGoogle Scholar
  18. 18.
    J. Minowada in.: “Human Lymphocyte Differentiation, its Implication to Cancer”, INSERM Symposium N°8, B. Serrou and C. Rosenfeld eds., Elsevier, Amsterdam, 337 (1978).Google Scholar
  19. 19.
    G.H. Reaman, J. Blatt and D.G. Poplack, Blood 58: 330 (1981).PubMedGoogle Scholar
  20. 20.
    G.R. Boss, L.F. Thompson, H.L. Spiegelberg, T.A. Waldmann, R. O’Connor, R.N. Hamburger and J.E. Seegmiller, Trans. Am. Ass. Physicians 92: 309 (1979).Google Scholar
  21. 21.
    J. Dornand, J.C. Bonnafous, C. Gavach and J.C. Mani, Biochimie 61: 973 (1979).PubMedCrossRefGoogle Scholar
  22. 22.
    J. Dornand, J.C. Bonnafous and J.C. Mani, FEBS Lett. 110: 34 (1980).CrossRefGoogle Scholar
  23. 23.
    J.C. Bonnafous, J. Dornand and J.C. Mani, FEBS Lett. 107: 95 (1979).PubMedCrossRefGoogle Scholar
  24. 24.
    J.C. Bonnafous, J. Favero, J. Dornand and J.C. Mani, J. Recept. Res. 2: 347 (1982)Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Jacques Dornand
    • 1
  • Jean-Claude Bonnafous
    • 1
  • Jean Favero
    • 1
  • Agnés Gartner
    • 1
  • Jean-Claude Mani
    • 1
  1. 1.Laboratoire de Biochimie des MembranesER CNRS 228 ENSCMMontpellierFrance

Personalised recommendations