Purine Metabolism in Rat Macrophages

  • Jerzy Barankiewicz
  • Amos Cohen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


The association of deficiencies of purine metabolic enzymes with immunodeficiency diseases has stimulated research in purine metabolism of lymphoid cells. Up to now our knowledge about purine metabolism of other cells of the immune system, e.g., macrophages, is relatively limited. During phagocytosis, macrophages excrete large amounts of uric acid1. The excretion of uric acid suggests that nucleotide catabolism may be predominant in phagocytosing macrophages. On the other hand, purine bases and nucleosides formed during digestion of nucleic acids may be transported across the phagolysosomal membrane and reutilized into intracellular nucleotide pools. Indeed activities of several purine salvage enzymes along with enzymes of purine catabolism have been found in macrophage extracts1,2.


Uric Acid Xanthine Oxidase Purine Metabolism Mouse Peritoneal Macrophage Purine Nucleoside Phosphorylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T.S. Chan, Purine excretion by mouse peritoneal macrophages lacking adenosine deaminase activity, Proc. Natl. Acad. Sci, USA 76: 925 (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    P.J. Edelson and Z. Cohn, 5′-nucleotidase activity of mouse peritoneal macrophages I. Synthesis and degradation in resident and inflamatory population, J. Exp. Med. 144: 1581 (1976).PubMedCrossRefGoogle Scholar
  3. 3.
    G.L. Tritsch and P.W. Niswander, Adenosine deaminase activity and superoxide formation during phagocytosis and membrane perturbation of macrophages, Immunol. Comm. 10: 1 (1981).Google Scholar
  4. 4.
    K. Yagawa and J. Okamura, Role of adenosine deaminase in activation of macrophages, Infect. Immunol. 32: 394 (1981).Google Scholar
  5. 5.
    J.F. Henderson, J.H. Fraser and E.E. McCoy, Methods for the study of purine metabolism in human cells in vitro, Clin. Biochem. 7: 339 (1974).PubMedCrossRefGoogle Scholar
  6. 6.
    A.S. Bognara, A.A. Letter and J.F. Henderson, Multiple mechanisms of regulation of purine biosynthesis de novo in intact tumor cells, Biochim. Biophys. Acta 374: 259 (1974).CrossRefGoogle Scholar
  7. 7.
    R.J. North, The concept of the activated macrophage, J. Immunol. 121: 806 (1978).PubMedGoogle Scholar
  8. 8.
    M.L. Karnovsky and J.K. Lazdins, Biochemical criteria for activated macrophages, J. Immunol. 121: 809 (1978).PubMedGoogle Scholar
  9. 9.
    Z.A. Cohn, The activation of a mononuclear phagocytes: Fact, fancy and future, J. Immunol. 121: 813 (1978).PubMedGoogle Scholar
  10. 10.
    C.F. Nathan, H.W. Murray and L.A. Cohn, The macrophages as an effector cell, N. Engl. J. Med. 303: 622 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    J. Michl, D.J. Ohlbaum and S.C. Silverstein, 2-Deoxyglucose selectively inhibts Fc and complement receptor-mediated phagocytosis in mouse peritoneal macrophages. I. Description of the inhibitory effect, J. Exp. Med. 144: 1465 (1976).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Jerzy Barankiewicz
    • 1
  • Amos Cohen
    • 1
  1. 1.Division of Immunology, Research InstituteThe Hospital for Sick ChildrenTorontoCanada

Personalised recommendations