Purine Synthesis During Amino Acid Starvation of Lymphoblasts With HPRT Deficiency or PP-Ribose-P Synthetase Overactivity Decreases Less Than in Normal Cells

  • Gerry R. Boss
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)


Normal human lymphoblastoid cells starved for 3 hours for any of several essential but not nonessential amino acids decrease their rates of purine nucleotide synthesis via the de novo and salvage pathways by 90% and 60%, respectively (1). Cycloheximide and puromycin each reduced de novo synthesis by 96% and salvage synthesis by 72%. The decrease in purine synthesis de novo is of first order kinetics and in the case of amino acid deprivation is fully and rapidly reversible after reconsititution of the amino acid (1).


Mutant Cell Methylene Tetrahydrofolate Salvage Pathway Normal Cell Line Amino Acid Starvation 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Gerry R. Boss
    • 1
  1. 1.Department of MedicineUniversity of CaliforniaSan Diego La JollaUSA

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