Biochemical and Immunological Evaluation of Long Term Coformycin Administration in the Mouse
The inherited human deficiency of adenosine deaminase is associated with severe combined immunodeficiency disease1,2. Coformycin is a potent inhibitor of adenosine deaminase and markedly potentiates the in vitro toxicity of adenosine and deoxyadenosine toward lymphoid cells. We wished to evaluate some of the biochemical and immunological events associated with coformycin administration in vivo. Increased dATP concentrations observed in adenosine deaminase deficiency3 are thought to inhibit ribonucleotide reductase and thereby restrict DNA synthesis. Another secondary consequence of a deficiency of adenosine deaminase may be inhibition of S-adenosylhomocysteine hydrolase by de oxyadenosine4.
KeywordsAdenosine Deaminase Severe Combine Immunodeficiency Severe Combine Immunodeficiency Disease Swiss Webster Mouse Pokeweed Mitogen
Unable to display preview. Download preview PDF.
- 5.P.P. Trotta, A. Tedde, S. Ikehara, R. Pahwa, R.A. Good, and M.E. Balis, Cancer Res. 41: 2189 (1979).Google Scholar
- 6.D.J. Nelson, S. Lafon, and C.U. Lambe, in: “Inborn Errors of Specific Immunity.” B. Pollara, R.J. Pickering, H.J. Meuwissen and I.H. Porter, ed., Academic Press, N.Y., p. 327, (1979).Google Scholar