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Decreased T Cell Ecto-5′-Nucleotidase Activity in Agammaglobulinemia Patients is Not Due to Altered Proportions of Helper and Suppressor T Cells as Defined by the Monoclonal Antibodies Okt4 and Okt8

  • Linda F. Thompson
  • Robert I. Fox
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

All patients with congenital X-linked agammaglobulinemia (XLA) and the majority of patients with common variable immunodeficiency (CVI) have reduced ecto-5′-nucleotidase (ecto-5′-NT) activity in their peripheral blood lymphocytes (1–3). Ecto-5′-NT deficiency in XLA and CVI patients may be partially explained by an absence of mature B lymphocytes. However, the majority of these patients also have decreased ecto-5′-NT activity in total T cell preparations. We have previously demonstrated that T? (suppressor) cells have only one third the ecto-5′-NT activity of Tμ (helper) cells (4); however, little is known regarding ecto-5′-NT activity in T cell subsets as defined by the monoclonal antibodies OKT4 (helper/inducer) and OKT8(cytotoxic/suppressor). Since many agammaglobulinemia patients have defects in T cell regulation of immunoglobulin biosynthesis, the experiments described here were designed to determine: (1) whether OKT4+ and OKT8+ cells isolated from normal individuals have different levels of ecto-5′-NT activity, and (2) whether low T cell ecto-5′-NT activity in XLA and CVI patients can be explained by altered proportions of subsets of regulatory T cells.

Keywords

Carbonyl Iron Common Variable Immunodeficiency Nucleotidase Activity Scripps Clinic Monoclonal Antibody OKT4 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Linda F. Thompson
    • 1
  • Robert I. Fox
    • 1
  1. 1.Departments of Immunopathology and Clinical ResearchScripps Clinic and Research FoundationLa JollaUSA

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