Decreased T Cell Ecto-5′-Nucleotidase Activity in Agammaglobulinemia Patients is Not Due to Altered Proportions of Helper and Suppressor T Cells as Defined by the Monoclonal Antibodies Okt4 and Okt8
All patients with congenital X-linked agammaglobulinemia (XLA) and the majority of patients with common variable immunodeficiency (CVI) have reduced ecto-5′-nucleotidase (ecto-5′-NT) activity in their peripheral blood lymphocytes (1–3). Ecto-5′-NT deficiency in XLA and CVI patients may be partially explained by an absence of mature B lymphocytes. However, the majority of these patients also have decreased ecto-5′-NT activity in total T cell preparations. We have previously demonstrated that T? (suppressor) cells have only one third the ecto-5′-NT activity of Tμ (helper) cells (4); however, little is known regarding ecto-5′-NT activity in T cell subsets as defined by the monoclonal antibodies OKT4 (helper/inducer) and OKT8(cytotoxic/suppressor). Since many agammaglobulinemia patients have defects in T cell regulation of immunoglobulin biosynthesis, the experiments described here were designed to determine: (1) whether OKT4+ and OKT8+ cells isolated from normal individuals have different levels of ecto-5′-NT activity, and (2) whether low T cell ecto-5′-NT activity in XLA and CVI patients can be explained by altered proportions of subsets of regulatory T cells.
KeywordsCarbonyl Iron Common Variable Immunodeficiency Nucleotidase Activity Scripps Clinic Monoclonal Antibody OKT4
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