Plasma Membrane 5′-Nucleotidase and other Purine Enzymes in Murine Lymphocytes
The important involvement of purine metabolism in human immune function has been established by the causal association of adenosine deaminase (ADA) and purine nucleoside Phosphorylase (PNP) deficiencies with severe immunodysfunction. Other purine enzymes have also been extensively examined for their potential roles in immune regulation. Such enzymes include: plasma membrane 5′-nucleotidase (5′-N), S-adenosylhomocysteine hydrolase (SAH-hydrolase), and hypoxanthine-guanine phosphoribosyltransferase (HPRT). The distribution of these purine enzymes has been investigated in subpopulations of lymphoid cells from humans1−5 and other vertebrates6−8. Many of the existing models of purine-induced lymphocytotoxicity9−10 center around the relative specific activities of these purine enzymes as a means of predicting which cells will be susceptible to the purine mediated cell killing. The lymphoid cells used in these model systems are almost uniformly human cells.
KeywordsAdenosine Deaminase Infectious Mononucleosis Purine Metabolism Purine Nucleoside Phosphorylase Thymus Cell
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