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Metabolism of Guanosine and Deoxyguanosine in Mammalian Lymphocytes and their Influence on Mitogenic Stimulation

  • G. J. Peters
  • A. Oosterhof
  • J. H. Veerkamp
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

PNP (purine nucleoside Phosphorylase) catalyzes the phosphoryly-sis of inosine, guanosine and their deoxycompounds. The substrates of PNP appear to play a key role in the expression of the disturbed immune function (1) and their concentrations are higher in erythrocytes, serum and urine of PNP deficient patients (2,3). An elevated concentration of dGTP was found in erythrocytes of these patients(2,3). B-cell lines are less sensitive to deoxyguanosine than T-cell lines (4). Human thymocytes are more sensitive to deoxyguanosine than peripheral lymphocytes (5–7). Deoxyguanosine phosphorylation in lymphoid cells is catalyzed by deoxycytidine kinase and by a specific deoxyguanosine kinase (8,9). The deoxyguanosine phosphorylating activity is high in thymus, spleen and peripheral lymphocytes (4,6,8-11). Since ovine lymphocytes (12) and rat thymocytes (13) have a very low PNP activity we studied kinetics of phosphorylation and phosphorylysis of inosine, guanosine and deoxyguanosine of these lymphoid cells and of human lymphocytes. The influence of inosine, guanosine and their deoxycompounds on mitogenic stimulation was also studied.

Keywords

Human Lymphocyte Peripheral Lymphocyte Purine Nucleoside Purine Nucleoside Phosphorylase Mitogenic Stimulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • G. J. Peters
    • 1
  • A. Oosterhof
    • 1
  • J. H. Veerkamp
    • 1
  1. 1.Department of BiochemistryUniversity of NijmegenNijmegenThe Netherlands

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