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Deoxyguanosine Kinase from Human Placenta

  • Yasukazu Yamada
  • Haruko Goto
  • Nobuaki Ogasawara
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 165)

Abstract

In the purine nucleoside Phosphorylase deficiency associated with immunodeficiency, the phosphorylation of deoxyguanosine to dGMP is important, since the disease is associated with an accumulation of dGTpl,2. Recently, some reports suggested the existence of distinct deoxyguanosine kinase (EC 2.7.4.8) from deoxycytidine kinase(EC 2.7.1.74) which also phosphorylated deoxyguanosine3-5. These studies showed that the phosphorylation of deoxyguanosine by deoxyguanosine kinase was different from that by deoxycytidine kinase in a Km value for deoxyguanosine and in the effects of nucleotides. We report several properties of deoxyguanosine kinase purified from human placenta.

Keywords

Human Placenta Purine Nucleoside Double Reciprocal Plot Purine Nucleoside Phosphorylase Deoxycytidine Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D. A. Carson, J. Kaye and J. E. Seegmiller, Lymphospecific toxicity in adenosine deaminase deficiency and purine nucleoside Phosphorylase deficiency: Possible role of nucleoside kinase, Proc. Natl. Acad. Sci. USA, 74: 5677 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    L. J. Gudas, B. Ullman, A. Cohen and D. W. Martin, Jr., Deoxy-guanosine toxicity in a mouse T-lymphoma: Relationship to purine nucleoside phosphorylase-associated immune dysfunction, Cell, 14: 531 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    W. R. Gower, Jr., M. C. Carr and D. H. Ives, Deoxyguanosine kinase: distinct molecular forms in mitochondria and cytosol, J. Biol. Chem., 254: 2180 (1979).PubMedGoogle Scholar
  4. 4.
    F. J. Green and R. A. Lewis, Partial purification and Characterization of deoxyguanosine kinase from pig skin, Biochem. J., 183: 547 (1979).PubMedGoogle Scholar
  5. 5.
    J. Barker and R. A. Lewis, Deoxyguanosine kinase of neonatal mouse skin tissue, Biochim. Biophys. Acta, 658: 111 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Yasukazu Yamada
    • 1
  • Haruko Goto
    • 1
  • Nobuaki Ogasawara
    • 1
  1. 1.Department of Biochemistry, Institute for Developmental ResearchAichi Prefectural ColonyKasugai, AichiJapan

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