Deoxyguanosine Kinase from Human Placenta
In the purine nucleoside Phosphorylase deficiency associated with immunodeficiency, the phosphorylation of deoxyguanosine to dGMP is important, since the disease is associated with an accumulation of dGTpl,2. Recently, some reports suggested the existence of distinct deoxyguanosine kinase (EC 18.104.22.168) from deoxycytidine kinase(EC 22.214.171.124) which also phosphorylated deoxyguanosine3-5. These studies showed that the phosphorylation of deoxyguanosine by deoxyguanosine kinase was different from that by deoxycytidine kinase in a Km value for deoxyguanosine and in the effects of nucleotides. We report several properties of deoxyguanosine kinase purified from human placenta.
KeywordsHuman Placenta Purine Nucleoside Double Reciprocal Plot Purine Nucleoside Phosphorylase Deoxycytidine Kinase
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