Laser Applications in Bio-Medicine: Tumor Therapy and Localization Using Photosensitizing Drugs

  • R. Cubeddu
  • R. Ramponi
  • C. A. Sacchi
  • O. Svelto
Part of the Ettore Majorana International Science Series book series (EMISS, volume 35)


Among the various applications of lasers in Biomedicine, a particularly interesting one makes use of a laser beam and photosensitizing drugs, for therapeutic and diagnostic purposes. Out of the several possibilities offered by this combination of laser light and drugs, we shall consider in this paper only the case of tumor therapy and tumor localization. The basic principles of these applications rely on the fact that a wide class of drugs present a high accumulation or retention in a tumor, or in a highly proliferative tissue, as compared with the surrounding normal tissue. For therapy the drug must also be able to induce a cytocidal reaction when activated by (laser) light of suitable wavelength. On the other side, for localization, when bound to the tissue, the drug must exhibit a reasonably high fluorescence quantum yield so that its emission can be detected by a suitable system. Thus it may happen that a drug suitable for tumor therapy is not suitable for tumor localization. In both these cases, however, two further conditions must be fulfilled:
  1. (i)

    - the drug must be nontoxic (in the dark) and non-mutagenic;

  2. (ii)

    - the drug must absorb and emit at wavelengths that can permit a reasonably good penetration into the tissues.



Photodynamic Therapy Distillation Column Tumor Therapy Excited Singlet State Copper Vapour Laser 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. Andreoni and R. Cubeddu (ed.s), “Porphyrins in Tumor Phototherapy”, Plenum Press, New York and London (1984)Google Scholar
  2. 2.
    G. Jori and C. Perria (ed.s), “Photodynamic Therapy of Tumors and Other Diseases”, Edizioni Libreria Progetto, Padova (1985)Google Scholar
  3. 3.
    D. Kessel (ed.), “Methods in Porphyrin Photosensitization”, Plenum Press, New York and London (1985)Google Scholar
  4. 4.
    Proc.s Clayton Foundation Conference on Photodynamic Therapy, Los Angeles, Ca, Feb. 15–19 (1987), Photochem. Photobiol., 46:561–952 (1987)Google Scholar
  5. 5.
    T. J. Dougherty, Photodynamic Therapy, in “Photodynamic Therapy of Tumors and Other Diseases”, G. Jori and C. Perria, ed.s, Edizioni Libreria Progetto, Padova (1985)Google Scholar
  6. 6.
    Y. Hayata, Photodynamic Therapy in Japan, in Proc.s 1st Int. Conference on the Clinical Applications of Photosensitization for Diagnosis and Treatment, Tokyo, Japan, April 30 — May 2, 1986Google Scholar
  7. 7.
    R. Cubeddu, W. F. Keir, R. Ramponi, T. G. Truscott, Photophysical Properties of Porphyrin-Chlorin Systems in the Presence of Surfactants, Photochem. Photobiol., 46:633 (1987)CrossRefGoogle Scholar
  8. 8.
    D. Kessel and C. J. Dutton, Photodynamic effects: Porphyrin vs chlorine, Photochem. Photobiol.; 40:403 (1984)CrossRefGoogle Scholar
  9. 9.
    E. Ben-Hur and I. Rosenthal, Photosensitized inactivation of Chinese Hamster cells by phtalocyanines, Photochem. Photobiol., 42:129 (1985)CrossRefGoogle Scholar
  10. 10.
    A. E. Profio, M. J. Carvlin, J. Sarnaik and L. R. Wudl, Fluorescence of Hematoporphyrin-Derivative for Detection and Characterization of Tumors in “Porphyrins and Tumor Phototherapy”, A. Andreoni and R. Cubeddu, ed.s, Plenum Press, New York and London (1984)Google Scholar
  11. 10a.
    J. P. A. Marijinissen, W. M. Star, J. L. van Delft and N. A. P. Franken, Light intensity measurements in optical phantoms and in vivo during HpD-Photoradiation treatment, using a miniature light detector with isotropic response in “Photodynamic Therapy of Tumors and Other Diseases”, G. Jori and C. Perria, ed.s, Edizioni Libreria Progetto, Padova (1985) A. Goetz, J. Feyh, P. Conzen and W. Brendel, In-vivo Detection of Hematoporphyrin Derivative (HpD) Fluorescence by Digital Subtraction of Videomicroscopic Images, Photochem. Photobiol., to be published.Google Scholar
  12. 11.
    A. Andreoni, R. Cubeddu and O. Svelto, Pulsed-Laser photoactivation of porphyrins in “Photodynamic Therapy of Tumors and Other Diseases”, G. Jori and C. Perria, ed.s, Libreria Progetto, Padova (1985)Google Scholar
  13. 12.
    R. Cubeddu, R. Ramponi and G. Bottiroli, Time-resolved fluorescence spectroscopy of hematoporphyrin derivative in micelles, Chem. Phys. Lett., 128:439 (1986)ADSCrossRefGoogle Scholar
  14. 13.
    R. Ramponi and M. A. J. Rodgers, An instrument for simultaneous acquisition of fluorescence spectra and fluorescence lifetimes from single cells, Photochem. Photobiol., 45:161 (1987)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • R. Cubeddu
    • 1
  • R. Ramponi
    • 1
  • C. A. Sacchi
    • 1
  • O. Svelto
    • 1
  1. 1.Centro di Elettronica Quantistica e Strumentazione Elettronica, CNRIstituto di Fisica del PolitecnicoMilanoItaly

Personalised recommendations