Effect of Intracerebroventricular Administered Vanadate on Salt and Water Intake and Excretion in the Rat

  • Emma Chiaraviglio
  • Constanza Lozada
Part of the NATO ASI Series book series (NSSA, volume 105)


The activity of sensors mediating thirst and ADH release has been correlated to NaCl concentration of the brain CSF. Substances known to inhibit active trans-membrane enzymatic Na transport have been found to inhibit water intake and ADH release (1,2), suggesting that active Na transport may be essential for the excitation of cerebral sensors involved in water balance. Therefore, the discovery that vanadate, a natural occurring substance, is a potent “in vitro” inhibitor of Na, K-ATPase activity (3) made it an interesting “tool” to study water balance. In rats, acute sodium depletion by peritoneal dialysis produced dramatic changes in serum and CSF sodium concentration (4,5). Furthermore infusion of hypertonic artificial CSF into the 3rd ventricle decreased Na intake induced by sodium depletion (5). Therefore it was of interest to study whether an inhibitor of enzymatic Na-transport such as vanadate, would affect sodium and water intake and excretion in conscious rats.


Peritoneal Dialysis Sodium Intake Sodium Depletion Salt Appetite Study Water Balance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. Rundgren, M.J. McKinley, L. G. Leksell and B. Andersson, Inhibition of thirst and apparent ADH release by intracerebroventricular ethacrinic acid, Acta physiol. scand. 105: 123 (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    R.S. Weisinger, D.A. Denton and M.J. McKinley, Inhibition of water intake byouabain administration in sheep, Pharmac.Biochem.Behav. 7: 121 (1977).CrossRefGoogle Scholar
  3. 3.
    L.C. Cantley, L. Josephson, R. Warner, M. Yanagisawa, C. Lechene and G. Guidotti, Vanadate is a potent (Na, K)-ATPase inhibitor found in ATP derived from muscle, J. Biol. Chem. 252: 7421 (1977).PubMedGoogle Scholar
  4. 4.
    M.C. Ferreyra and E. Chiaraviglio, Changes in volemia and natremia and onset of sodium appetite in sodium depleted rats, Physiol. Behav. 19: 197 (1977).PubMedCrossRefGoogle Scholar
  5. 5.
    E. Chiaraviglio and M.F. Perez Guaita, Effect of cerebroventricular infusion of hypertonic sodium solutions on sodium intake in the rat, This Symposium.Google Scholar
  6. 6.
    J.J. Grantham; The renal sodium pump and vanadate, Am. J. Physiol. 239:F97 (1980).PubMedGoogle Scholar
  7. 7.
    E. Chiaraviglio and M.F. Perez Guaita, Antero third ventricle (A3V) lesion and homeostasis regulation, Symposium on Body Fluid Homeostasis, Evian (1983).Google Scholar
  8. 8.
    D.A. Denton, F.W. Kaintz, The inhibition of salt appetite of sodium deficient sheep by intracarotid infusion of ouabain, Comm.Behav. Biol. 4: 183 (1969).Google Scholar
  9. 9.
    B. Appelgren and S. Ericksson, Apparent inhibition of the ADH secretion induced by intracerebroventricular vanadate, Acta physiol. scand. 108:C18 (1980).Google Scholar
  10. 10.
    W.E. Balfour and A.L.R. Findlay, Intracerebroventricular vanadate and natriuresis in the rat, J. Physiol. 322:35P (1982).Google Scholar
  11. 11.
    W.E. Balfour, J.J. Grantham and I.M. Glynn, Vanadate stimulate natriuresis, Nature, London, 275: 768 (1978).CrossRefGoogle Scholar
  12. 12.
    L. Beaugé, Vanadate-Potassium interactions in the inhibition of Na, K-ATPase. From: “Na, K-ATPase: Structure and Function”. Skou, J.C. and Norby, J. Editors, Academic Press, New York, pp. 373 (1979).Google Scholar
  13. 13.
    L. Beaugé and I.M. Glynn, Commercial ATP containing traces of vanadate alters the response of (Na+ + K+)ATPase to external potassium, Nature, London, 272: 551 (1978).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Emma Chiaraviglio
    • 1
  • Constanza Lozada
    • 1
  1. 1.Instituto de Investigaciòn MédicaMercedes y Martìn FerreyraCòrdobaArgentina

Personalised recommendations