The Plasticity of Mature Oligodendrocytes: A Role for Substratum in Phenotype Expression
Non — attached. To achieve this, oligodendrocytes are seeded into tissue culture plates where they form floating clusters. Biochemically, morphologically and ultrastructurally they resemble freshly isolated cells. We call these cells B3.f OLG. B3 for band III oligodendrocytes; f for floating; (Szuchet et. al., 1980). Routinely we keep B3.f OLG for 4–5 days, but extending this time to 16 days does not alter the metabolism of the cells, nor is there any obvious change in morphology (Szuchet and Yim, 1984; Yim et. al., 1986).
Attached. When B3.f OLG (after 4 to 5 days in vitro) are transferred to polylysine coated plates, they adhere within 24h and develop a profuse network of processes. We refer to these cells as B3.fA OLG (A for adherent). We have maintained B3.fA OLG for 120 days with better than 98% purity as assessed by immuno-cytochemical criteria (Fig. 1).
KeywordsMyelin Basic Protein Vasoactive Intestinal Peptide Vasoactive Intestinal Polypeptide Mature Oligodendrocyte Myelin Membrane
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- Arvanitis, D. and Szuchet, S., 1986, Myelin palingenesis, Abstract, Second Joint Meeting of World Federation of Neurology, Baden, Vienna, August 28–30.Google Scholar
- Arvanitis, D., Szuchet, S., Polak, P. E., and Yim, S. H., 1986, Molecular organization of myelin membranes, J. Cell Biol., 103, 229a.Google Scholar
- Benjamins, J. A., 1984, Protein metabolism of oligodendroglial cells in vivo, in; “Oligodendroglia”, Plenum Press, New York.Google Scholar
- Morell, P., Toews, A. D., 1984, In vivo metabolism of oligodendroglial lipids, in: “Oligodendroglia”, Plenum Press, New York.Google Scholar
- Peters, A., Palay, S. L., Webster, H. D. F., 1976, in: “The fine structure of the nervous system”, Saunders, Philadelphia.Google Scholar
- Sarliève, L. L., Fabre, M., Rebel, G., Susz, J., Vincendon, G., Matthieu, J. M., 1983a, Myelin-like or pre-myelin structures in cultures of dissociated brain cells from 14–15 day-old embryonic mice, in: “Protides of the biological fluids”, Pergamon Press, Oxford.Google Scholar
- Szuchet, S., 1987, Myelin palingenesis: the reformation of myelin by mature oligodendrocytes in the absence of neurons, in: “Glial-Neuronal Communication in Development and Regeneration”, A. Althaus and W. Seifert, eds., Springer-Verlag, New York.Google Scholar
- Szuchet, S., Polak, P. E., and Yim, S. H., 1986, Mature oligodendrocytes cultured in the absence of neurons recapitulate the ontogenic development of myelin, Dev. Neurosci., 208–221.Google Scholar
- Szuchet, S., Polak, P. E., Yim, S. H., and Arvanitis, D., 1987, The plasma membrane of living oligodendrocytes. III. Relatedness to myelin. Submitted.Google Scholar
- Szuchet, S., Stefnsson, K., Wollmann, R. L., Dawson, G., and Arnason, B. G. W., 1980, Maintenance of isolated oligodendrocytes in long-term culture, Brain Res., 200–151.Google Scholar
- Vartanian, T., 1987, PhD, Thesis, University of Chicago, Chicago, IL.Google Scholar
- Vartanian, T., Szuchet, S., and Dawson, G., 1987, Oligodendrocyte substratum adhesion modulates expression of adenylate cyclase linked receptors, Submitted for publication.Google Scholar
- Vartanian, T., Yim, S. H., Szuchet, S., and Dawson, G., 1986b, Synthesis and phosphorylation of myelin basic protein in oligodendrocyte cultures: regulatory events, Adv. Biosci., 61: 133.Google Scholar
- Yim, S. H., Szuchet, S., and Polak, P. E., 1986, Cultured oligodendrocytes, J. Biol. Chem., 261: 11808.Google Scholar