Quantitative Assay of Dopamine Receptor Subtypes
Dopamine, acting as a neurotransmitter in the central nervous system, is involved in the regulation of a variety of behaviors in man and animals. Studies carried out over the last decade have shown that not all of the responses attributed to the release of dopamine are mediated through the same kind of receptor. Cools and Van Rossum (1976) first proposed the existence of two types of receptors for dopamine in an attempt to explain results obtained in studies of the effects of dopamine and apomorphine on stereotypy and locomotor activity. However, precise pharmacological characterization of receptors is difficult if only behavioral models are utilized. Biochemical studies have shown that stimulation of dopamine receptors can result, in some tissues, in an increase in the accumulation of cyclic AMP through activation of dopamine-sensitive adenylate cyclase (Kebabian and Greengard, 1971). Not all dopamine receptors, however, appear to act through increases in cyclic AMP levels.
KeywordsFrontal Cortex Dopamine Receptor Guanine Nucleotide Adenylate Cyclase Activity Hill Coefficient
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