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Colon pp 103-119 | Cite as

Bacteriology of the Colon

  • Gary L. Simon
  • Sherwood L. Gorbach
Part of the Topics in Gastroenterology book series (TGEN)

Abstract

The microflora of the colon is an extraordinarly complex ecologic niche, consisting of both aerobic and anaerobic bacteria. Extensive microbiologic studies have revealed that a single individual harbors more than 400 different bacterial species in the colonic flora.1 Characterization of such a heterogenous mixture by classic bacteriologic techniques is a monumental task; it has been estimated that a complete microbiologic analysis of a single fecal specimen requires up to 1 year to complete. Fortunately, a reasonable description of the colonic microflora is possible to construct by considering only the most representative microorganisms (Table 1).

Keywords

Natl Cancer Inst Intestinal Microflora Fecal Flora Colonic Flora Germfree Animal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Moore WEC, Holdeman LV: Discussion of current bacteriologic investigations of the relationships between intestinal flora, diet, and colon cancer. Cancer Res 35:3418–3420, 1975.Google Scholar
  2. 2.
    Donaldson Jr, RM: Normal bacterial populations of the intestine and their relation to intestinal function. N Engl J Med 270:938–945, 994–1001, 1050–1056, 1964.PubMedGoogle Scholar
  3. 3.
    Moore WEC, Holdeman LV: Human fecal flora; the normal flora of 20 Japanese-Hawaiians. Appl Microbiol 27:961–979, 1974.PubMedGoogle Scholar
  4. 4.
    Donaldson Jr, RM: The relation of enteric bacterial population to gastrointestinal function and disease, in Sleisenger MH, Fordtran JS (eds): Gastrointestinal Disease, Philadelphia, WB Saunders Co, 1978, pp 79–92.Google Scholar
  5. 5.
    Finegold SM, Sutter VL, Sugihara PT et al: Fecal microbial flora in Seventh Day Adventist populations and control subjects. Am J Clin Nutr 30:1781–1792, 1977.PubMedGoogle Scholar
  6. 6.
    Gorbach SL: Intestinal microflora. Gastroenterology 60:1110–1129, 1971.PubMedGoogle Scholar
  7. 7.
    Gorbach SL, Nahas L, Lerner PI et al: Studies of intestinal microflora. I. Effects of diet, age, and periodic sampling on numbers of fecal microorganisms in man. Gastroenterology 53:845–855, 1967.PubMedGoogle Scholar
  8. 8.
    Hill MJ, Drasar BS: The normal colonic bacterial flora. Gut 16:318–323, 1975.PubMedGoogle Scholar
  9. 9.
    Fitzgerald JF: Colonization of the gastrointestinal tract, in: Mead Johnson Symposium on Perinatal and Developmental Medicine. Selected Aspects of Perinatal Gastroenterology. Mead Johnson Symposium on Perinatal and Developmental Medicine. 1977, pp 35–38.Google Scholar
  10. 10.
    Long SS, Swenson RM: Development of anaerobic fecal flora in healthy newborn infants. J Pediatr 91:298–301, 1977.PubMedGoogle Scholar
  11. 11.
    Bishop RF, Anderson CM: The bacterial flora of the stomach and small intestine in children with intestinal obstruction. Arch Dis Child: 35:487, 1960.PubMedGoogle Scholar
  12. 12.
    Bentley DW, Nichols RL, Condon RE et al: The microflora of the human ileum and intraabdominal colon: Results of direct needle aspiration at surgery and evaluation of the technique. J Lab Clin Med 79:421–429, 1972.PubMedGoogle Scholar
  13. 13.
    Kalser NH, Cohen R, Arteaga I et al: Normal viral and bacterial flora of the human small and large intestine. N Engl J Med 274:500–505, 1966.PubMedGoogle Scholar
  14. 14.
    Clarke RTJ: Methods for studying gut microbes, in Clarke RTJ, Bauchop T (eds): Microbial Ecology of the Gut. New York, Academic Press Inc, 1977, pp 1–33.Google Scholar
  15. 15.
    Drasar BS: Cultivation of anaerobic intestinal bacteria. J Pathol Bacteriol 94:417–427, 1967.PubMedGoogle Scholar
  16. 16.
    Holdeman LV, Moore WEC: Anaerobe Laboratory Manual. Blacksburg, Virginia Polytechnic Institute, 1972.Google Scholar
  17. 17.
    Cherry WB, Moss CW: The role of gas chromatography in the clinical microbiological laboratory. J Infect Dis 119:658–662, 1969.PubMedGoogle Scholar
  18. 18.
    Gorbach SL, Mayhew JW, Bartlett JG et al: Rapid diagnosis of anaerobic infections by direct gasliquid chromatography of clinical specimens. J Clin Invest 57:478–484, 1976.PubMedGoogle Scholar
  19. 19.
    Gorbach SL, Levitan R: Intestinal flora in health and in gastrointestinal diseases, in Glass GBJ (ed): Progress in Gastroenterology. New York, Grune & Stratton Inc, vol 2, 1970, pp 252–275.Google Scholar
  20. 20.
    Dack GM, Petran E: Bacterial activity in different levels of the intestine and in isolated segments of small and large bowel in monkeys and dogs. J Infect Dis 54:204–220, 1934.Google Scholar
  21. 21.
    Dixon JMS: The fate of bacteria in the small intestine. J Pathol Bacteriol 79:131–140, 1960.PubMedGoogle Scholar
  22. 22.
    Drasar BS, Hill MJ: Human Intestinal Flora. New York, Academic Press Inc, 1974.Google Scholar
  23. 23.
    Broido PW, Gorbach SL, Nyhus LM: Microflora of the gastrointestinal tract and the surgical malabsorption syndromes. Surg Gynecol Obstet. 135:449–460, 1972.PubMedGoogle Scholar
  24. 24.
    Donaldson RM Jr: Small bowel bacterial overgrowth. Adv Int Med 16:191–212, 1970.Google Scholar
  25. 25.
    Drasar BS, Shiner M: Studies on the intestinal flora. II. Bacterial flora of the small intestine in patients with gastrointestinal disorders. Gut 10:812–819, 1969.PubMedGoogle Scholar
  26. 26.
    Gracey M: The contaminated small bowel syndrome: pathogenesis, diagnosis, and treatment. Am J Clin Nutr 32:234–243, 1979.PubMedGoogle Scholar
  27. 27.
    Gorbach SL, Tabaqchali S: Bacteria, bile, and the small bowel. Gut 10:963–972, 1969.PubMedGoogle Scholar
  28. 28.
    King CE, Toskes PP: Small intestine bacterial overgrowth. Gastroenterology 76:341–348, 1979.Google Scholar
  29. 29.
    Luckey TD: Bicentennial overview of intestinal microecology. Am J Clin Nutr 30:1753–1761, 1977.PubMedGoogle Scholar
  30. 30.
    Savage DC: Microbial ecology of the gastrointestinal tract. Ann Rev Microbiol 31:107–133, 1977.Google Scholar
  31. 31.
    Wolin MJ: Metabolic interactions among intestinal microorganisms. Am J Clin Nutr 27:1320–1328, 1974.PubMedGoogle Scholar
  32. 32.
    Byrne BM, Dankert J: Volatile fatty acids and aerobic flora in the gastrointestinal tract of mice under various conditions. Infect Immun 23:559–563, 1979.PubMedGoogle Scholar
  33. 33.
    Gorbach SL, Spanknebel G, Weinstein L et al: Studies of intestinal microflora. VIII. Effect of lincomycin on the microbial population of the human intestine. J Infect Dis 120:298–304, 1969.PubMedGoogle Scholar
  34. 34.
    Barlett JG, Chang TW, Gurwith M et al: Antibiotic-associated Pseudomembranous colitis due to toxin producing Clostridia. N Engl J Med 298:531–534, 1978.Google Scholar
  35. 35.
    Nichols RL, Broido P, Condon RE et al: Effect of preoperative neomycin-erythromycin intestinal preparation on the incidence of infectious complications following colon cancer. Ann Surg 178:453–462, 1973.PubMedGoogle Scholar
  36. 36.
    Nichols RL, Condon RE, Gorbach SL et al: Efficacy of properative antimicrobial preparation of the bowel. Ann Surg 176:227–232, 1972.PubMedGoogle Scholar
  37. 37.
    Bartlett, JG, Chang TW, Taylor NS et al: Colitis induced by Clostridium difficile. Rev Infect Dis 1:370–378, 1976.Google Scholar
  38. 38.
    Bornside GH, Cohn Jr I: Stability of normal human fecal flora during a chemically defined, low residue liquid diet. Ann Surg 181:58–60, 1974.Google Scholar
  39. 39.
    Bounous G, Devroede GJ: Effects of an elemental diet on human fecal flora. Gastroenterology 66:210–214, 1974.PubMedGoogle Scholar
  40. 40.
    Attebery HR, Sutter VL, Finegold SM: Effect of a partially chemically defined diet on normal human fecal flora. Am J Clin Nutr 25:1391–1398, 1972.PubMedGoogle Scholar
  41. 41.
    Gorbach SL: The effect of diet on the intestinal microflora and its metabolic functions, in Shils ME (ed): Defined formula Diets for Medical Purposes. Chicago, American Medical Association, 1977.Google Scholar
  42. 42.
    Maier BR, Flynn MA, Burton GC et al: Effects of a high-beef diet on bowel flora: A preliminary report. Am J Clin Nutr 27:1470–1474, 1974.PubMedGoogle Scholar
  43. 43.
    Hentges DJ: Fecal flora of volunteers on controlled diets: Am J Clin Nutr 31.S123–S124, 1978.PubMedGoogle Scholar
  44. 44.
    Cohen R, Kalser MH, Arteaga I et al: Microbial intestinal flora in acute diarrheal disease. JAMA 201:835–840, 1967.PubMedGoogle Scholar
  45. 45.
    Gorbach SL, Banwell JG, Jacobs B et al: Intestinal microflora in Asiatic cholera. I. “Rice-water” stool. J Infect Dis 121:32–37, 1970.PubMedGoogle Scholar
  46. 46.
    Gorbach SL, Banwell JG, Jacobs B et al: Intestinal microflora in Asiatic cholera. II. The small bowel. J Infect Dis 121:38–45, 1970.PubMedGoogle Scholar
  47. 47.
    Gorbach SL, Neale G, Levitan R et al: Alterations in human intestinal microflora during experimental diarrhea. Gut 11:1–6, 1970.PubMedGoogle Scholar
  48. 48.
    Gorbach SL, Banwell JG, Chatterjee BD et al: Acute undiferrentiated human diarrhea in the tropics. I. Alterations in intestinal microflora. J Clin Invest 50:881–889, 1971.PubMedGoogle Scholar
  49. 49.
    Gorbach SL, Kean BH, Evans DG et al: Traveler’s diarrhea and toxigenic Escherichia coli. N Engl J Med 292:933–936, 1975.PubMedGoogle Scholar
  50. 50.
    Gorbach SL, Khurana CM: Toxigenic Escherichia coli: A cause of infantile diarrhea in Chicago. N Engl J Med 287:791–795, 1972.PubMedGoogle Scholar
  51. 51.
    Guerrant RL, Moore RA, Kirschenfeld PM et al: Role of toxigenic and invasive bacteria in acute diarrhea of childhood: N Engl J Med 293:567–573, 1975.PubMedGoogle Scholar
  52. 52.
    Merson MH, Morris GK, Sack DA et al: Travelers’ diarrhea in Mexico. A prospective study of physicians and family members attending a Congress. N Engl J Med 294:1299–1304, 1976.PubMedGoogle Scholar
  53. 53.
    Ryder RW, Wachsmuth K, Buxton AE et al: Infantile diarrhea produced by heat-stabile enterotoxigenic Escherichia coli. N Engl J Med 295:849–853, 1976.PubMedGoogle Scholar
  54. 54.
    Dubos R, Schaedler RW, Costello R et al: Indigenous, normal, and autochthonous flora of the gastrointestinal tract. J Exp Med 122:67–77, 1965.PubMedGoogle Scholar
  55. 55.
    Savage DC: Interactions between the host and its microbes, in Clarke RTJ, Bauchop T (eds): Microbial Ecology of the Gut. New York, Academic Press Inc, 1977, pp 277–310.Google Scholar
  56. 56.
    Savage DC, Dubos R, Schaedler RW: The gastrointestinal epithelium and its autochthonous bacterial flora. J Exp Med 127:67–75, 1967.Google Scholar
  57. 57.
    Gorbach SL: Recombinant DNA: An infectious disease perspective. J Infect Dis: 136:615–623, 1978.Google Scholar
  58. 58.
    Cooke EM, Ewins SP, Shooter R: The changing fecal population of E. coli in hospital medical patients. Br Med J 4:593–595, 1969.PubMedGoogle Scholar
  59. 59.
    Sears HI, Brownlee I, Uchiyama JK: Persistence of individual strains of E coli in the intestinal tract of man. J Bacteriol 59:293–301, 1950.PubMedGoogle Scholar
  60. 60.
    Sears HT, Brownlee I: Further observations on the persistence of individual strains of E. coli in the intestinal tract of man. J Bacteriol 63:47–57, 1952.PubMedGoogle Scholar
  61. 61.
    Smith HW: Transfer of antibiotic resistance from animal and human strains of E. coli in the alimentary tract of man. Lancet 1:1174–1176, 1969.PubMedGoogle Scholar
  62. 62.
    Formal SB, Hornick RB: Invasive Escherichia coli. J Infect Dis 137:641–644, 1978.PubMedGoogle Scholar
  63. 63.
    Abrams GD: Microbial effects on mucosal structure and function. Am J Clin Nutr 30:1880–1886, 1977.PubMedGoogle Scholar
  64. 64.
    Abrams GD, Bauer H, Sprinz H: Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. A comparison of germ-free and conventional mice. Lab Invest 12:355–364, 1963.PubMedGoogle Scholar
  65. 65.
    Coates ME, Fuller B: The gnotobiotic animal in the study of gut microbiology, in Clarke RTJ, Bauchop T (eds): Microbial Ecology of the Gut. New York, Academic Press Inc, 1977, pp 311–346.Google Scholar
  66. 66.
    Thompson GR, Trexler PC: Gastrointestinal structure and function in germ-free or gnotobiotic animals. Gut 12:230–235, 1971.PubMedGoogle Scholar
  67. 67.
    Gordon HA, Bruchorer-Kardoss E: Effect of nomal microbial flora on intestinal surface area. Am J Physiol 201:175–182, 1961.PubMedGoogle Scholar
  68. 68.
    Lesher S, Walburg HE, Sacher GA: Generation cycle in the duodenal crypt cells of germ-free and conventional mice. Nature (London) 202:884–886, 1964.Google Scholar
  69. 69.
    Kenworthy R: Observations on the reaction of the intestinal mucosa to bacterial challenge. J Clin Pathol 24:138–145, 1971.Google Scholar
  70. 70.
    Klipstein FA, Goetsch CA, Engert RF et al: Effect of monocontamination of germ-free rats by enterotoxigenic coliform bacteria. Gastroenterology 76:341–348, 1979.PubMedGoogle Scholar
  71. 71.
    Gracey M, Papadimitrious J, Bower G: Ultrastructural changes in the small intestines of rats with selffilling blind loops. Gastroenterology 67:646–651, 1974.PubMedGoogle Scholar
  72. 72.
    Toskes PP, Giannella RA, Jervis HR et al: Small intestine mucosal injury in the experimental blind loop syndrome. Gastroenterology 68:1193–1203, 1975.PubMedGoogle Scholar
  73. 73.
    Gordon HA, in Coates ME (ed): The Germfree Animal in Research. New York, Academic Press Inc, 1968, pp 127–150.Google Scholar
  74. 74.
    Gordon HA, Pesti L: The gnobiotic animal as a tool in the study of host microbial relationships. Bacteriol Rev 35:390–429, 1971.PubMedGoogle Scholar
  75. 75.
    Skelly BJ, Trexter PC, Tanami J: Effect of a Clostridium species upon cecal size of gonobiotic mice. Proc Soc Exp Biol 110:455–458, 1962.Google Scholar
  76. 76.
    Gorbach SL, in Finegold SM (ed): Management of Anaerobic Infections. Ann Int Med 83:375–389, 1975.Google Scholar
  77. 77.
    Weinstein WM, Onderdonk AB, Bartlett JG et al: Experimental intra-abdominal abcesses in rats: Development of an experimental model. Infect Immun 10:1250–1255, 1974.PubMedGoogle Scholar
  78. 78.
    Onderdonk AB, Weinstein WM, Sullivan NM et al: Experimental intra-abdominal abscesses in rats: Quantitative bacteriology of infected animals. Infect Immun 19:1256–1259, 1974.Google Scholar
  79. 79.
    Weinstein WM, Onderdonk AB, Bartlett JG et al: Antimicrobial therapy of experimental intraabdominal sepsis. J Infect Dis. 132:282–286, 1975.PubMedGoogle Scholar
  80. 80.
    Tally FP: Determinants of virulence in anaerobic bacteria. Microbiology 1979:219–223, 1979.Google Scholar
  81. 81.
    Tally FP, Stewart PR, Sutter VL et al: Oxygen tolerance of fresh clinical anaerobic bacteria. J Clin Microbiol 1:161–164, 1975.PubMedGoogle Scholar
  82. 82.
    Tally FP, Goldin BR, Jacobus NV et al: Superoxide dimutase in anaerobic bacteria of clinical significance. Infect Immun 16:20–25, 1977.PubMedGoogle Scholar
  83. 83.
    Kasper DL: The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: Immunochemical and morphological definition. J Infect Dis 138:79–87, 1976.Google Scholar
  84. 84.
    Simon GL, Klempner MSJ, Kasper DL et al: Alterations in opsonophagocytic killing by neutrophils of Bacteroides fragilis associated with animal and laboratory passage: Effect of capsular polysaccharide. J Inf Dis 145:72–77, 1982.Google Scholar
  85. 85.
    Kasper DL, Onderdonk AB, Polk BF et al: Surface antigens as virulence factors in infection with Bacteroides fragilis. Rev Infect Dis 1:278–288, 1979.PubMedGoogle Scholar
  86. 86.
    Onderdonk AB, Kasper DL, Cisneros RL et al: The capsular polysaccharide of Bacteroides fragilis as a virulence factor: Comparison of the pathogenic potential of encapsulated and unencapsulated strains. J Infect Dis 136:82–89, 1977.PubMedGoogle Scholar
  87. 87.
    Finegold SM, Attebery HR, Sutter VL: Effect of diet on human fecal flora: Comparison of Japanese and American diets. Am J Clin Nutr 27:1456–1469, 1974.PubMedGoogle Scholar
  88. 88.
    Kasper DL, Hayes ME, Reinap BG et al: Isolation and identification of encapsulated strains of Bacteroides fragilis. J Infect Dis 136:75–81, 1977.PubMedGoogle Scholar
  89. 89.
    Polk BF, Kasper DL: Bacteroides fragilis subspecies in clinical isolates. Ann Int Med 86:569–571, 1977.PubMedGoogle Scholar
  90. 90.
    Armstrong B, and Doll R: Environmental factors and cancer incidence and mortality in different countries, with special references to dietary practices. Int J Cancer 15:617–631, 1975.PubMedGoogle Scholar
  91. 91.
    Burkitt DP: Epidemiology of cancer of the colon and rectum. Cancer 28:3–13, 1971.PubMedGoogle Scholar
  92. 92.
    Doll R: The geographical distribution of cancer. Br J Cancer 23:1–8, 1969.PubMedGoogle Scholar
  93. 93.
    Drasar BS, Irving D: Environmental factors and cancer of the colon and breast. Br J Cancer 27:167–172, 1973.PubMedGoogle Scholar
  94. 94.
    Wynder EL: The epidemiology of large bowel cancer. Cancer Res 35:3388–3394, 1975.PubMedGoogle Scholar
  95. 95.
    Wynder EL, Kajitani T, Ischikawa S et al: Environmental factors of cancer of the colon and rectum. Cancer 23:1210–1220, 1969.PubMedGoogle Scholar
  96. 96.
    Crowther JS, Drasar BS, Hill MJ et al: Faecal steroids and bacteria and large bowel cancer in Hong Kong by socioeconomic groups. J Cancer 34:191–198, 1976.Google Scholar
  97. 97.
    Haenszel W, Berg JW, Segi M et al: Large-bowel cancer in Hawaiian Japanese: J Natl Cancer Inst. 51:1765–1779, 1973.PubMedGoogle Scholar
  98. 98.
    Hill MJ, Drasar BS, Aries V et al: Bacteria and aetiology of cancer of large bowel. Lancet 1:95–100, 1971.PubMedGoogle Scholar
  99. 99.
    Finegold SM, Sutter VL: Fecal flora in different populations, with special reference to diet. Am J Clin Nutr 31:S116–S122, 1978.PubMedGoogle Scholar
  100. 100.
    Hill MJ: Bacteria and the etiology of colonic cancer. Cancer 34:815–818, 1974.PubMedGoogle Scholar
  101. 101.
    Weisburger JH: Colon carcinogenes: Their metabolism and mode of action. Cancer 28:60–70, 1971.PubMedGoogle Scholar
  102. 102.
    Goldin BR, Gorbach SL: The relationship between diet and rat fecal bacterial enzymes implicated in colon cancer. J Natl Cancer Inst 57:371–375, 1976.PubMedGoogle Scholar
  103. 103.
    Scheline RR: Metabolism of foreign compounds by gastrointestinal microorganisms. Pharmacol Rev 25:451–523, 1973.PubMedGoogle Scholar
  104. 104.
    Laqueur GL, Mickelson O, Whitting MG et al: Carcinogenic properties of nuts from Cyclos circonilis. J Natl Cancer Inst 31:919–951, 1963.PubMedGoogle Scholar
  105. 105.
    Laqueur GL, Spatz M: Toxicology of cycasin. Cancer Res 28:2262–2267, 1968.PubMedGoogle Scholar
  106. 106.
    Laqueur GL: The indication of intestinal neoplasia with the glycoside of cycasin and its aglycone. Virchows Arch Pathol Anat Physiol 340:151–163, 1965.Google Scholar
  107. 107.
    Laqueur GL, McDaniel EG, Matsumoto H: Tumor induction in germ-free rats with methylazoxymethanol (MAM) and synthetic MAM acetate. J Natl Cancer Inst 39:355–371, 1967.PubMedGoogle Scholar
  108. 108.
    Gillette JR, Kamm JJ, Sasame HA: Mechanism of p-nitro-benzoate reduction in mice: The possible role of cytochrome P-450 in liver microsomes. Mol Pharmacol 4:541–548, 1968.PubMedGoogle Scholar
  109. 109.
    Kato R, Oshima R, Takanara A: Studies on the mechanism of nitro reduction by rat liver. Mol Pharmacol 5:487–494, 1962.Google Scholar
  110. 110.
    Hill MJ: The role of colon anaerobes in the metabolism of bile acids and Steroides, and its relation to colon cancer. Cancer 36:2387–2400, 1975.PubMedGoogle Scholar
  111. 111.
    Mastromarino A, Reddy BS, Wynder EL: Metabolic epidemiology of colon cancer: Enzymic activity of fecal flora. Am J Clin Nutr 29:1455–1460, 1976.PubMedGoogle Scholar
  112. 112.
    Reddy BS, Mastromarino A, Wynder EL: Further leads on metabolic epidemiology of large bowel cancer. Cancer Res 35:3403–3406, 1975.PubMedGoogle Scholar
  113. 113.
    Reddy BS, Weisburger JH, Wynder EL: Effects of dietary fat level and dimethylhydrazine on fecal acid and neutral sterol excretion and colon carcinogenesis in rats. J Natl Cancer Inst 52:507–511, 1974.PubMedGoogle Scholar
  114. 114.
    Reddy BS, Wynder EL: Large-bowel carcinogenesis: Fecal bacterial constituents of populations with diverse incidence rates of colon cancer. J Natl Cancer Inst 50:1437–1442, 1973.PubMedGoogle Scholar
  115. 115.
    Mower HF, Ray RM, Shoff R et al: Fecal bile acids in two Japanese populations with different colon cancer risks. Cancer Res 39:328–331, 1979.PubMedGoogle Scholar
  116. 116.
    Reddy BS, Weisburger JH, Wynder EL: Fecal bacterial-glucuronidase: Contol by diet. Science 183:416–417, 1974.PubMedGoogle Scholar
  117. 117.
    Granthame PH, Horton RE, Weisburger EK, Weisburger JH: Metabolism of the carcinogen N-2-fluorenylacetamide in germ free and conventional rats. Biochem Pharmacol 19:163–171, 1974.Google Scholar
  118. 118.
    Weisburger JH Granthame PM, Horton RE, Weisburger EK: Metabolism of the carcinogen N-hydroxy-N-2-fluorenzlacetamide in germ free rats. Biochem Pharmacol 19:151–162, 1970.PubMedGoogle Scholar
  119. 119.
    Goldin B, Dwyer J, Gorbach SL et al: Influence of diet and age on fecal bacterial enzymes. Am J Clin Nutr 31:S136–140, 1978.PubMedGoogle Scholar
  120. 120.
    Goldin BR: The role of diet and the intestinal flora in the etiology of large bowel cancer. International Symposium on Colorectal Cancer, abstracted, New York, 1979.Google Scholar
  121. 121.
    Reddy BS, Narisawa T, Wright P et al: Colon carcinogenesis with azoxymethane and dimethylhydrazine in germ free rats. Cancer Res 35:287–290, 1975.PubMedGoogle Scholar
  122. 122.
    Goldin BR, Gorbach SL: The effect of Lactobacillus acidophilus dietary supplements on DMH induced colon cancer in rats. J Natl Cancer Inst 64:263–266, 1980.PubMedGoogle Scholar
  123. 123.
    Goldin BR, Swenson L, Dwyer J et al: Effect of diet and Lactobacillus supplements on human fecal bacterial enzymes. J Natl Cancer Inst 64:255–262, 1980.PubMedGoogle Scholar
  124. 124.
    Metchnikoff E: The Prolongation of Life. Putnam Press (eds): New York, 1908.Google Scholar
  125. 125.
    Beck C, and Necheles H: Beneficial effects of administration of Lactobacillus acidophilus in diarrheal and other disorders. Am J Gastroenterol 35:522–530, 1961.PubMedGoogle Scholar
  126. 126.
    Rettger LF, Levy MN, Weinstein L et al: Lactobacillus acidophilus and its therapeutic applications New Haven, Conn, Yale University Press, 1935.Google Scholar
  127. 127.
    Paul D, Hoskins LC: Effect of oral lactobacillus feedings on fecal lactobacillus counts. Am J Clin Nutr 25:763–765, 1972.PubMedGoogle Scholar
  128. 128.
    Report from the International Agency for Research on Cancer. Intestinal microecology group: Dietary fiber, transit-time, fecal bacteria, steroids and colon cancer in two Scandinavian populations. Lancet 2:207–211, 1977.Google Scholar
  129. 129.
    Goldin BR, Gorbach SL: Alterations in fecal microflora enzymes related to diet, age, Lactobacillus supplements and dimethylhydrazine. Cancer 40:2421–2426, 1977.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Gary L. Simon
    • 1
    • 2
  • Sherwood L. Gorbach
    • 1
    • 2
  1. 1.Division of Infectious DiseasesThe George Washington University Medical CenterUSA
  2. 2.Division of Infectious DiseasesTufts-New England Medical CenterBostonUSA

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