Ubiquitin: A Newly Identified Component of Paired Helical Filaments

  • Hiroshi Mori
  • Jun Kondo
  • Yasuo Ihara
Chapter

Abstract

The progressive accumulation of unusual fibers in neuronal perikarya and processes characterizes the brain affected with Alzheimer’s disease (AD). The fibers are composed of two 10 nm filaments wound into a helix with a half-periodicity of 80 nm and thus termed paired helical filaments (PHF)1.

Keywords

Cellulose HPLC Urea Glycine Carboxyl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. Kidd, Paired helical filaments in electron microscopy of Alzheimer’s disease. Nature 197:192 (1963).CrossRefGoogle Scholar
  2. 2.
    D. J. Selkoe, Y. Ihara, and F. Salazar, Alzheimer’s disease: Insolubility of partially purified paired helical filements in sodium dodecyl sulfate and urea. Science 215:1243 (1982).CrossRefGoogle Scholar
  3. 3.
    Y. Ihara, C. Abraham, and D. J. Selkoe, Antibodies to paired helical filaments in Alzheimer’s disease do not recognize normal brain proteins. Nature 304:727 (1983).CrossRefGoogle Scholar
  4. 4.
    N. Nukina, and Y. Ihara, One of the antigenic determinants of paired helical filaments is related to tau protein. J. Biochem. 99:1541 (1986).Google Scholar
  5. 5.
    I. Grundke-Iqbal, K. Iqbal, M. Quinlan, Y. C. Tung, M. S. Zaidi, and H. M. Wisniewski, Microtubule-associated protein tau: a component of Alzheimer paired helical filaments. J. Biol. Chem. 261:6084 (1986).Google Scholar
  6. 6.
    H. Mori, J. Kondo, and Y. Ihara, Ubiquitin is a component of paired helical filaments in Alzheimer’s disease. Science 235:1641 (1987).CrossRefGoogle Scholar
  7. 7.
    T. Masaki, T. Fujihashi, and M. Soejima, Effect of various inhibitors on the activity of Achromobacter Protease I. Nippon Nogeikagaku Kaishi 58:865 (1984).CrossRefGoogle Scholar
  8. 8.
    A. L. Haas, and P. M. Bright, The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates. J. Biol. Chem. 260:12464 (1985).Google Scholar
  9. 9.
    A. Hershko, Ubiquitin: Roles in protein modification and breakdown. Cell 34:11 (1983).CrossRefGoogle Scholar
  10. 10.
    J. Ananthan, A. L. Goldberg, and R. Voellmy, Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes. Science 232:522 (1986).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Hiroshi Mori
    • 1
  • Jun Kondo
    • 1
    • 2
  • Yasuo Ihara
    • 1
  1. 1.Tokyo Metropolitan Institute of GerontologyItabashiku Tokyo 173Japan
  2. 2.Mitsubishi Chemical Industries Limited Research CenterBiosciences LaboratoryKamoshida, Midoriku Yokohama 227Japan

Personalised recommendations