Immunohistochemical Study of Alzheimer Disease Using Antibodies to Synthetic Amyloid and Fibronectin
In order to know whether or not fibronectin, an adhesive glycoprotein, is related to the amyloid deposition in the senile plaque, we conducted immunohistochemical studies using polyclonal anti-fibronectin and affinitypurified anti-amyloid component (Affi 28). Affi 28 was made by immunizing a rabbit against the synthetic peptide corresponding to residues 1–28 of the amyloid core protein reported by Masters et al (1985). In the brain sections of Alzheimer disease confirmed by Affi 28, the senile plaques were not stained by anti-fibronectin. So, it became clear that there was no association between the amyloid in the senile plaque and fibronectin at least immunohistochemically. The absence of fibronectin in the senile plaque suggests that fibronectin may not be requested for the deposition of amyloid fibrils. In addition, we described that not only senile plaques and cerebrovascular amyloid but also subpial regions were stained by Affi 28.
KeywordsAlzheimer Disease Down Syndrome Amyloid Plaque Senile Plaque Amyloid Fibril
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