β-Amyloid cDNA Cloned from Alzheimer’s Disease Brain
A cDNA clone for the presumed precursor for the α-amyloid peptide (BAP) has been isolated from an Alzheimer’s disease (AD) brain library. It encompasses up to 60% of the full length mRNA open reading frame proposed by Kang et al. (1) to encode the normal BAP precursor (BAPP). The AD clone has nucleotide sequences identical to BAPP precursor cDNAs cloned from normal human adult brain (2) and fetus (1). This indicates that there are no mutations in or about the 42 amino acid sequence of the BAP which is deposited in AD brain lesions. An antisense radiolabelled RNA copy of one of the AD clones detects three gene transcripts of J.5, J.2 and 1.6 kilobases (kb). All three transcripts are present in normal human and AD brain RNAs. These data suggest that the accumulation of amyloid consistently found in AD brains is not likely the result of a mutation in the AD-BAPP gene.
KeywordsFull Length mRNA BAPP Gene Putative Glycosylation Site Normal Human Adult Brain Onal Aging
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