Preparation of a Monoclonal Antibody Against a Synthetic Brain Amyloid Beta Peptide(N1–10) and Distribution of an Immunoreactivity in Serum

  • Tomotaka Shinoda
  • Yoshie Kametani
  • Tetsuya Hiroe
  • Kazuso Iinuma
  • Kazuo Miyanaga


Alzheimer’s disease(AD) is a progressive neurodegenerative disorder of the human central nervous system characterized by formation of neurofibrillary tangles(NFT), senile(neuritic) plaques, and cerebrovascular amyloid-osis1,2,3. The mature senile plaques(SP) consist of degenerating neurites surrounding a core(SPC) of proteinaceous filaments which have structural properties of amyloid. Similar filaments are also found in the walls of meningeal and intracortical blood vessels(CVA). The initial isolation and partial sequencing of a 4.2kD polypeptide(called β protein or A4 peptide) reported for amyloid isolated from extraparenchymal meningeal vessels in AD aswell as in Down’s syndrome(DS)4 and, subsequently, from SPC. These findings suggesting a common origin for both types of amyloid, may also indicate that a common or a very similar mechanism may involve in the formation of amyloid in AD and DS. Recently, the base sequence has been determined by several laboratories of a cDNA for a precursor amyloid β protein6,7 Subsequently, the gene has been mapped in the vicinity of theSOD locus on chromosome 21. The putative precursor of amyloid β protein consists of 695 amino acid residues and has a structure characteristic of a cell surface receptor.


Polyclonal Antiserum Western Blot Technique Progressive Neurodegenerative Disorder Normal Seron Rabbit Serum Albumin 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Tomotaka Shinoda
    • 1
  • Yoshie Kametani
    • 1
  • Tetsuya Hiroe
    • 1
  • Kazuso Iinuma
    • 2
  • Kazuo Miyanaga
    • 3
  1. 1.Department of ChemistryTokyo Metropolitan UniversityTokyo 158Japan
  2. 2.National Children’s HospitalTokyo 154Japan
  3. 3.Department of PsychoneurologyGunma University School of MedicineMaebashiJapan

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