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Preparation of a Monoclonal Antibody Against a Synthetic Brain Amyloid Beta Peptide(N1–10) and Distribution of an Immunoreactivity in Serum

  • Tomotaka Shinoda
  • Yoshie Kametani
  • Tetsuya Hiroe
  • Kazuso Iinuma
  • Kazuo Miyanaga
Chapter

Abstract

Alzheimer’s disease(AD) is a progressive neurodegenerative disorder of the human central nervous system characterized by formation of neurofibrillary tangles(NFT), senile(neuritic) plaques, and cerebrovascular amyloid-osis1,2,3. The mature senile plaques(SP) consist of degenerating neurites surrounding a core(SPC) of proteinaceous filaments which have structural properties of amyloid. Similar filaments are also found in the walls of meningeal and intracortical blood vessels(CVA). The initial isolation and partial sequencing of a 4.2kD polypeptide(called β protein or A4 peptide) reported for amyloid isolated from extraparenchymal meningeal vessels in AD aswell as in Down’s syndrome(DS)4 and, subsequently, from SPC. These findings suggesting a common origin for both types of amyloid, may also indicate that a common or a very similar mechanism may involve in the formation of amyloid in AD and DS. Recently, the base sequence has been determined by several laboratories of a cDNA for a precursor amyloid β protein6,7 Subsequently, the gene has been mapped in the vicinity of theSOD locus on chromosome 21. The putative precursor of amyloid β protein consists of 695 amino acid residues and has a structure characteristic of a cell surface receptor.

Keywords

Polyclonal Antiserum Western Blot Technique Progressive Neurodegenerative Disorder Normal Seron Rabbit Serum Albumin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Tomotaka Shinoda
    • 1
  • Yoshie Kametani
    • 1
  • Tetsuya Hiroe
    • 1
  • Kazuso Iinuma
    • 2
  • Kazuo Miyanaga
    • 3
  1. 1.Department of ChemistryTokyo Metropolitan UniversityTokyo 158Japan
  2. 2.National Children’s HospitalTokyo 154Japan
  3. 3.Department of PsychoneurologyGunma University School of MedicineMaebashiJapan

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