A Unique Protein that Shares a Common Epitope with Senile Plaque in Alzheimer’s Disease Brain
Senile plaque is one of the most characteristic chemical and pathological hallmarks in the brain of patients with Alzheimer’s disease (AD)(1,2). It has already been described that immunoglobulin (3), complement(4), and β-protein(5), are immunochemically detected as components which construct senile plaques in this pathological condition. Accordingly, identification of such proteins will be of crucial importance in clarifying the pathogenesis of this condition. We have previously reported that a monoclonal antibody(Hy-20-54--16-3L) preferentially recognized senile plaques in AD brain (6). This antibody was one of the monoclonal antibodies which were obtained by immunizing the mouse with a crude protein preparation from amyloid laden spleen of primary amyloidosis(6). Recently with immunoblotting technique, we found a protein which was crossreacted by the monoclonal antibody in a 23,000 x g supernatant fraction of AD brain(7). This protein, named SPRP(senile plaque-related protein), seems to share a common epitope with the constituents in senile plaques. The characterization of the protein may be of Importance to clarify the pathogenesis of senile plaques. We now report some biochemical characteristics of this protein.
KeywordsSenile Plaque Common Epitope Immunochemical Staining Primary Amyloidosis Cerebral Grey Matter
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