Cloning and Sequence Analysis of cDNAs Encoding Mouse C-Reactive Protein
The primary structure of mouse C-reactive protein (CRP) has been determined from a complementary DNA (cDNA) clone apparently for the first time. It consists of 225 amino acids, including the entire CRP sequence (205 amino acids) and a putative signal sequence (20 amino acids). When the deduced amino acid sequence of mouse CRP was compared with that of human CRP, a 70% homology was evident.
KeywordsDeduce Amino Acid Sequence Serum Amyloid cDNA Insert Amino Acid Sequence Homology Primary Amino Acid Sequence
Unable to display preview. Download preview PDF.
- 1.A. P. Osmand, B. Friedenson, H. Gewurz, R. H. Painter, T. Hofmann, and E. Shelton, Characterization of C-reactive protein and the complement subcomponent Clt as homologous proteins displaying cyclic pentameric symmetry (pentraxins), Proc. Natl. Acad. Sci. USA 74:739–743 (1977).CrossRefGoogle Scholar
- 4.M. B. Pepys, A. C. Dash, R. E. Markham, H. C. Thomas, B. D. Williams, and A. Petrie, Comparative clinical study of protein SAP (amyloid P component) and C-reactive protein in serum, Clin. Exp. Immunol. 32:119–124 (1978).Google Scholar
- 5.F. C. de Beer, M. L. Baltz, E. A. Munn, A. Feinstein, J. Taylor, C. Bruton, J. R. Clamp, and M. B. Pepys, Isolation and characterization of C-reactive protein and serum amyloid P component in the rat, Immunology 45:55–70 (1982).Google Scholar
- 6.S. Ohnishi, S. Maeda, K. Shimada. and T. Arao, Isolation and characterization of the complete complementary and genomic DNA sequences of human serum amyloid P component, J. Biochem. 100:849–858 (1986).Google Scholar
- 7.K.-J. Lei, T. Liu, G. Zon, E. Sorabia, T.-Y. Liu, and N. D. Goldman, Genomic DNA sequence for human C-reactive protein. J. Biol. Chem. 260:13377–13383 (1985).Google Scholar
- 8.P. Woo, J. R. Korenberg, and A. S. Whitehead, Characterization of genomic and complementary DNA sequence of human C-reactive protein, and comparison with the complementary DNA sequence of serum amyloid P component, J. Biol. Chem. 260:13384–13388 (1985).Google Scholar
- 9.E. C. Mantzouranis, S. B. Dowton, A. S. Whitehead, M. D. Edge, G. A. P. Bruns, and H. R. Colten, Human serum amyloid P component, J. Biol. Chem. 260:7752–7756 (1985).Google Scholar
- 10.C. Syin, E. C. Gotschlich, and T.-Y. Liu, Rabbit C-reactive protein, J. Biol. Chem. 261:5473–5479 (1986).Google Scholar
- 12.S. Wakasugi, S. Maeda, K. Shimada. H. Nakashima, and S. Migita, Structural comparisons between mouse and human prealbumin, J. Biochem. 98:1707–1714 (1985).Google Scholar
- 15.T. Maniatis, E. F. Fritsch, and J. Sambrook, “Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory, NY (1982).Google Scholar
- 19.J. A. Taylor, C. J. Bruton, J. K. Anderson, J. E. Mole, F. C. de Beer, L. Baltz, and M. B. Pepys, Amino acid sequence homology between rat and human C-reactive protein, Biochem. J. 221:903–906 (1984).Google Scholar